Abstract
Lignocellulosic materials derived from forages, namely timothy grass, alfalfa, reed canary grass, and agricultural residues, such as corn stalks and barley straw, were pretreated using ammonia fiber explosion (AFEX) process. The pretreated materials were directly saccharified by cellulolytic enzymes. Sixty to 80% of theoretical yield of sugars were obtained from the pretreated biomasses. Subsequent ethanolic fermentation of the hydrolysates byPachysolen tannophilus ATCC 32691 resulted in 40-60% of theoretical yield after 24 h, based on the sugars present in the hydrolysates. The uptake of sugars was not complete, indicating a possible inhibitory effect onP. tannophilus during the fermentation of these substrates.
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Alvo, P., Savoie, P., Tremblay, D., Émond, J. P., and Turcotte, G. (1996),Bioresource Technol. 56, 61–68.
Stone, J. E., Scallan, A. M., Danefer, E., and Ahlgren, E. (1969),Adv. Chem. Serv. 95, 219–223.
Fan, L. T., Lee, Y. H., and Gharpuray, M. M. (1982),Adv. Biochem. Eng. Biotechnol. 23, 157–187.
Fan, L. T., Lee, Y. H., and Beardmore, D. H. (1980),Biotechnol. Bioeng. 22, 177–199.
Sinitsyn, A. P., Gusakov, A. V., and Vlasenko, E. Y. (1991),Appl. Biochem. Biotechnol. 30, 43–59.
Himmel, M., Tucker, M., Baker, J., Rivard, C., Oh, K., and Grohmann, K. (1985),Biotechnol. Bioeng. Symp. 15, 39–58.
Millett, M. A., Baker, A. J., and Satter, L. D. (1976),Biotechnol. Bioeng. Symp. 6, 125–153.
Brownell, H. H. and Saddler, J. N. (1984),Biotechnol. Bioeng. Symp. 14, 55–68.
Chua, M. G. S. and Wayman, M. (1979),Can. J. Chem. 57, 1141–1146.
Shultz, T. P., Biermann, C. J., and Mc Ginnis, G. D. (1983),Ind. Eng. Chem. Prod. Res. Dev. 22, 344–348.
Mackie, K. L., Brownell, H. H., West, K. L., and Saddler, J. N. (1985),J. Wood Chem. Tech. 5/3, 405–425.
Belkacemi, K., Turcotte, G., Savoie, P., and Chornet, E. (1997),Ind. Eng. Chem. Res. 36, 4572–4580.
Iyer, P. V., Wu, Z. W., Kim, S. B., and Yoon, Y. L. (1996),Appl. Biochem. Biotechnol. 57/58, 121–132.
Yoon, H. H., Wu, Z. W., and Lee, Y. Y. (1994),Appl. Biochem. Biotechnol. 51/52, 5–19.
Kim, S. B. and Lee, Y. Y. (1996),Appl. Biochem. Biotechnol. 57/58, 147–156.
Dale, B. E. and Moreira, M. J. (1982),Biotechnol. Bioeng. Symp. 12, 31–43.
Bothast, R. J., Dien, B. S., Iten, L. B., Hespell, R. B., and Lawton, J. W. (1996), inLiquid Fuels and Industrial Products from Renewable Resources, Proceedings of the Liquid Fuel Conference, Nashville, TN, ASAE St-Joseph, pp. 241–252.
Dale, B. E. and de la Rosa, L. (1992), inLiquid Fuels from Renewable Resources, Proceedings of an Alternative Energy Conference, Nashville, TN, ASAE St-Joseph, pp. 162–170.
Dale, B. E., Leong, C. K., Pham, T. K., Esquivel, V. M., Rios, I., and Larimer, V. M. (1994), inLiquid Fuels, Lubricants and Additives from Biomass, Proceedings of an Alternative Energy Conference, Nashville, TN, ASAE St-Joseph, pp. 104–111.
de la Rosa, L., Reshamwala, S., Larimer, V. M., Shawky, B. T., Dale, B. E., and Stuwart, E. D. (1994),Appl. Biochem. Biotechnol. 45/46, 483–497.
Holzapple, M. T., Jun, J. H., Ashok, G., Patibandla, S. L., and Dale, B. E. (1991),Appl. Biochem. Biotechnol. 28/29, 59–74.
Holzapple, M. T., Ripley, E. P., and Nikolaou, M. (1994),Biotechnol. Bioeng. 44, 1122–1131.
Moniruzzaman, M., Dien, B. S., Ferrer, B., Hespell, R. B., Dale, B. E., Ingram, L. O., and Bothast, R. J. (1996),Biotechnol. Lett. 18, 985–990.
Miller, G. L. (1959),Anal. Chem. 31, 426–428.
Belkacemi, K., Turcotte, G., de Halleux, D., and Savoie, P. (1996), inLiquid Fuels and Industrial Products from Renewable Resources, Proceedings of the Liquid Fuel Conference, Nashville, TN, ASAE St-Joseph, pp 232–240.
Chiquette, J. (1997), inProceedings of the 1997 Ethanol Research and Development Workshop, Ottawa, ON, pp 111–114.
Beck, M. J. (1986),Biotechnol Bioeng. Symp. 17, 615–627.
Coughlan, M. P. (1985),Biotechnol Gen. Eng. Rev. 3, 39–109.
Ramos, L. P., Breuil, C., and Saddler, J. N. (1993),Enzyme Microbiol. Technol. 15, 19–25.
Saddler, J. N. (1986),Microbiol. Sci. 3, 84–87.
Wood, T. M. (1989), inEnzymes Systems for Lignocellulose Degradation, Coughlan, M. P., ed., Elsevier, London, pp. 17–35.
Christov, L. and Prior, B. (1993),Enzyme Microbiol. Technol. 15, 460–475.
Penner, M. H. and Liaw, E. T. (1994), inEnzymatic Conversion of Biomass for Fuels Production, Himmel, M. E., Baker, J. O., and Overend, R. P., eds., ACS Symposium Series 566, pp. 363–371.
Panchal, C. J., Bast, L, Russell, I., and Stewart, G. G. (1988),Can. J. Microbiol. 34, 1316–1320.
Dubus, D., Methner, H., Shulze, D., and Dellweg, H. (1983),Eur. J Appl. Microbiol. Biotechnol. 17, 287–291.
Jeffries, T. W. (1990), inYeast Biotechnology and Biocatalysis, Verachtert, H. and De Mot, R., eds. Marcel Dekker, Louvain, Belgium, pp. 349–393.
Dien, B. S., Kurtzman, C. P., Saha, B. C., and Bothast, R. J. (1996),Appl. Biochem. Biotechnol. 57/58, 233–242.
Ligthelm, M. E., Prior, B. A., and Du Preez, J. C. (1988),Appl. Microbiol. Biotechnol. 28, 293–296.
Wilkie, K. C. B. (1979), inAdvances Carbohydrate Chemistry and Biochemistry, Vol. 36 Tipson, R. S., and Horton, D., eds., Academic, New York pp. 215–264.
Alvo, P. and Belkacemi, K. (1997),Bioresource Technol. in press.
Fan, L. T., Gharpuray, M. M., and Lee, Y. H. (1987), inCellulose Hydrolysis, Springer-Verlag, New York, pp. 5–120.
Poutanen, K. and Puls, J. (1989), inBiogenesis and Biodegradation of Plant Cell Wall Polymers, Lewis, G. and Paice, M., eds., American Chemical Society, Washington D. C., pp. 456–467.
Doran, J. B. and Ingram, L. O. (1993),Biotechnol. Prog. 9, 533–538.
Ohta, K., Beall, D. S., Mejia, J. P., Shanmugam, K. T., and Ingram, L. O. (1991),Appl. Environ. Microbiol. 57, 893–900.
Takahashi, D. F., Carvalhal, M. L., and Alterthum, F. (1994),Biotechnol. Lett. 16, 747–750.
York, S. W. and Ingram, L. O. (1996),Biotechnol. Lett. 18, 683–688.
Zhang, M., Eddy, C., Deanda, K., Finkelstein, M., and Picataggio, S. (1995),Science 267, 240–243.
Schneider, H., Wang, P. Y., Chan, Y. K., and Maleska, R. (1981),Biotechnol. Lett. 3, 89–92.
Slininger, P. J., Bothast, R. J., van Cauwenberge, J. E., and Kurtzman, C. P. (1982),Biotechnol. Bioeng. 24, 371–384.
Slininger, P. J., Bolen, P. L., and Kurtzman, C. P. (1987),Enzyme Microbiol. Technol. 9, 5–15.
Delgenes, J. P., Moletta, R., and Navarro, J. M. (1986),Biotechnol. Lett. 8, 897–900.
Woods, M. A. and Millis, N. F. (1985),Biotechnol. Lett. 7, 679–682.
Du Preez, J. C. and Prior, B. A. (1985),Biotechnol. Lett. 7, 241–246.
Olsson, L. and Hahn-Hägerdal, B. (1996),Enzyme Microbiol. Technol. 18, 312–331.
Zhang, M., Eddy, C., Deanda, K., Finkelstein, M., and Picataggio, S. (1995),Science 267, 240.
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Belkacemi, K., Turcotte, G., Halleux, D.d. et al. Ethanol production from AFEX-treated forages and agricultural residues. Appl Biochem Biotechnol 70, 441–462 (1998). https://doi.org/10.1007/BF02920159
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DOI: https://doi.org/10.1007/BF02920159