Summary
To investigate therapeutic efficiency of Ad/CMV-hTGF-β1 gene for rabbit intervertebral dise degeneration model. 60 Japanese white rabbits were selected to form the L5-L6 Anterior-Lateral-Anulus-Fibrosus-Incision-Induced model in order to simulate human intervertebral dise degeneration. 36 rabbits, whose corresponding intervertebral discs were injected with 20 μl (10×106 pfu) of Ad CMV-hTGF-β1 gene, constituted the therapy group, 12 were injected with 20 μl (10×106 pfu) of Ad CMV-LacZ gene as comparison group, while 12 were only injected with equivalent capacity of saline for empty comparison group, 3 weeks after injection, examples were taken for investigation of HE staining, MRI. Western Blotting and immunohistochemical research TGF-β1. Wide distribution of TGF-β1 was detected by immunohistochemical research in the degenerated annulus fibrosus after injection. Western Blotting research showed significant increase of TGF-β1 content in intervertebral dises treated with TGF-β1 gene than comparison groups. MRI signal transformed from low to comparatively high and that intervertebral dise pathological degree improved. Ad CMV-hTGF-β1 gene transfection is a potential method to increase TGF-β1 content and reverse intervertebral dise degeneration.
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ZHAN Zirui, male, born in 1970 Associate Professor
The Project Sponsored by the Scientific Research Foundation for the Returned Overseas Chinese Scholars, Ministry of Education P.R.C. (No. [2001]345).
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Zirui, Z., Zengwu, S., Xiaoquian, X. et al. Ad/CMV-hTGF-β1 treats rabbit intervertebral discs degenerationin vivo. Current Medical Science 24, 599–601 (2004). https://doi.org/10.1007/BF02911367
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DOI: https://doi.org/10.1007/BF02911367