Abstract
We present here microwave-based modifications of standard protein assays that dramatically reduce the time required to determine protein concentrations. Typical protein determinations involve incubation times ranging from 15–60 min. Microwave irradiation of specimens reduces this time requirement to 10–20 s without compromising accuracy or reliability. The remarkable speed with which protein determinations may be carried out using microwave enhancement greatly simplifies general laboratory procedures that depend on the estimation of protein concentrations.
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Akins, R. E. and Tuan, R. S. (1992) Measurement of protein in 20 seconds using a microwave BCA assay.Biotechniques 12, 496–499.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951) Protein measurement with the Folin phenol reagent.J. Biol. Chem. 193, 65–275.
Smith, P. K., Krohn, R. I., Hermanson, G. T., Mallia, A. K., Gartner, F. H., Provenzano, M. D., Fujimoto, E. K., Goeke, N. M., Olson, B. J., and Klenk, D.C. (1985) Measurement of protein using bicinchoninic acid.Analyt. Biochem. 150, 76–85.
Kok, L. P. and Boon, M. E. (1992)Microwave Cook-book for Microscopists. Coulomb, Leyden, Leiden, Netherlands.
Warburg, O. and Christian, W. (1941) Isolierung und kristallisation des garunsferments enolase.Biochemische Zeitschrift 310, 384–421.
Layne, E. (1957) Spectrophotometric and turbidimetric methods for measuring proteins.Meth. Enzymol. 3, 447–454.
Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Analyt. Biochem. 72, 248–254.
Gornall, A. G., Bardawill, C. J., and David, M. M. (1949) Determination of serum proteins by means of the biuret reaction.J. Biol. Chem. 177, 751.
Dawson, R. M. C., Elliott, D. C., Elliott, W. H., and Jones, K. M. (1969)Data for Biochemical Research. 2nd ed. Oxford University Press, London, p. 618.
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An erratum to this article is available at http://dx.doi.org/10.1007/BF02921620.
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Akins, R.E., Tuan, R.S. Ultrafast protein determinations using microwave enhancement. Mol Biotechnol 4, 17–24 (1995). https://doi.org/10.1007/BF02907468
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DOI: https://doi.org/10.1007/BF02907468