Abstract
The content of phenolic substances in the cell suspension culture ofCentaurium erythraea fluctuated during a 21-day-long subcultivation period in dependence on the growth phase. The total relative content of the phenolics reached its maximum at the time of transition to the exponential growth phase, similarly as the fraction of free phenolic acids, glycosides, and the fraction of phenolic acids released from the cells after alcaline hydrolysis. On the other hand, the content of phenolic acid esters decreased at this growth phase of the culture. Changes in the level of phenolic substances in the culture medium corresponded in their character to changes in the relative content of the phenolics in the cells.
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Constabel, F.,Shyluk, J. P.,Gamborg, O. L..: The effect of hormones on anthocyanin accumulation in cell cultures ofHaplopappus gracilis. - Planta96: 306–316, 1971.
Beggs, Ch. J., Stolzer-jehle, A., Welimans, E.: Isoflavonoid formation as an indicator of UV stress in bean(Phaseolus vulgaris L.) leaves. - Plant Physiol.79: 630–634, 1985.
Berlin, J., Babz, W.: Oxidative decarboxylation of para-hydroxybenzoic acids underin vivo andin vitro conditions. - Z. Naturforsch.30: 650–658, 1975.
Davies, M. E.: Polyphenol synthesis in cell suspension cultures of Paul’s Scarlet rose. - Planta104: 50–65, 1972.
Friend, J.: Phenolic substances and plant disease. In: Swain, T.,Harborne, J. vanSumeee, C. F. (ed.): Recent Advances in Biochemistry. Vol. 12. Biochemistry of Plant Phenolics. Pp. 567–588. Plenum Press, New York 1979.
Hahlbrock, K.,Schröder, J.,Vieregge, J.: Enzyme regulation in parsley and soybean cell cultures. - In:Fiechter, A. (ed.): Plant Cell Cultures II. Pp. 39–60. Akademie-Verlag, Berlin 1981.
Hahlbrock, K., Wellmann, E.: Light-induced flavone biosynthesis and activity of phenylalanine ammonia-lyase and UDP-apiose synthetase in cell suspension cultures ofPetroselinum hortense. - Planta94: 236–239, 1970.
Hattori, T.,Ohta, Y.: Induction of phenylalanine ammonia-lyase activation and isoflavone glucoside accumulation in suspension-cultured cells of red bean,Vigna angularis, by phytoalexin elicitors, vanadate, and elevation of medium pH. - Plant Cell Physiol.26: 1101–1110, 1985.
Kojima, M.,Uritani, I.: Studies on chlorogenic acid biosynthesis in sweet potato root tissue using trans-cinnamic aeid-2-14C and quinic acid-G-3H. - Plant Cell Physiol.13: 311–319, 1972.
Meravy, L.: Phenolic substances in tissue cultures ofCentaurium erythraea. Biol. Plant.29: 81–87, 1987.
Sahai, O. P., Shuler, M. L.: Environmental parameters influencing phenolics production by batch cultures ofNicotiana tabacum. - Biotechnol. Bioeng.26: 111–120, 1984.
Tanaka, Y., Uritani, I.: Effect of auxin and other hormones on the metabolic response to wounding in sweet potato roots. -. Plant Cell Physiol.20: 1557–1564, 1979.
Yamakawa, T., Kato, S., Ishida, K., Kodama, T., Minoda, Y.: Production of anthocyanins byVitis cells in suspension culture. - Agr. biol. Chem.47: 2185–2191, 1983.
Zadernowski, R., Kozlowska, H.: Phenolic acids in soybean and rapeseed flours. - Lebensm.-Wiss.-Technol.16: 110–114, 1983.
Zádor, E., Kovbs, E., Szabo, M.: Phenolic materials in auxin heterotroph and autotroph (habituated) tobacco callus tissues.- Biochem. Physiol. Pflanzen180: 125–131, 1985.
Zaprometov, M. N.: [Phenolic compounds and the methods of their investigation.] In Russ. -In:Pavlinova, O. A. (ed.): Biokhimicheskie Metody v Fiziologii Rastenii. [Biochemical Methods in Plant Physiology.] - Nauka, Moskva 1971.
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Meravý, L. Phenolic substances in the cell suspension culture ofCentaurium erythraea . Biol Plant 29, 247–252 (1987). https://doi.org/10.1007/BF02892784
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DOI: https://doi.org/10.1007/BF02892784