Summary
Supernatants (SN) from 10 phytohemagglutinin (PHA)-stimulated human T cell clones (TCC), selected for their helper function on IgE synthesis, were found to provide IgE helper activity in atopic B cells showing low or undetectable spontaneousin vitro IgE synthesis. In contrast, SN from 5 PHA-stimulated TCC unable to provide helper function for IgE synthesis consistently failed to elicit IgE production. SN active on IgE synthesis contained high concentrations of interleukin-4 (IL-4), whereas inactive SN did not contain detectable amounts of IL-4. Moreover, the IgE helper activity of TCC SN was strongly inhibited by the addition of interferon-γ (IFN-γ) to B cell cultures. These data suggest that IL-4 may play a role in the induction ofin vitro human IgE synthesis, whereas IFN-γ displays an inhibitory effect.
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These studies were supported by grants from theMinistero della Pubblica Istruzione (contract № 12.02.01355) and from theConsiglio Nazionale delle Ricerche (CNR), Roma, Italy (contract № 87.01508).
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Maggi, E., Del Prete, G.F., Tiri, A. et al. Role of interleukin-4 in the induction of human IgE synthesis and its suppression by interferon-γ. La Ricerca Clin. Lab. 17, 363–367 (1987). https://doi.org/10.1007/BF02886920
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DOI: https://doi.org/10.1007/BF02886920