Abstract
The latex agglutination test (LAT) and enzyme-linked immunosorbent assay (ELISA) were evaluated in separate studies for their ability to detect potato virus X (PVX) and potato virus S (PVS) in tissue culture plantlets. Healthy and infected clonal lines of several potato cultivars were used. LAT was unsatisfactory because only low levels of agglutination were obtained with infected samples, and because variable and inconsistent results were obtained with both healthy and infected clones. ELISA, however, consistently gave high spectrophotometric readings and intense visual reactions for infected but not for healthy clones. The results indicate that ELISA can be used to detect PVX and PVS in tissue culture plantlets, and in programs where tissue culture is employed, early detection and elimination of infected plantlets is possible.
Resumen
En estudios separados se evaluaron la prueba de aglutinación con Latex (LAT) y la de inmunoabsorción con conjugados enzimáticos (ELISA) con el fin de determinar su capacidad para detectar los viruses X y S de la papa en plántulas de cultivo de tejidos. Se utilizaron líneas clonales sanas e infectadas de varios cultivares de papa. La prueba LAT no fue satisfactoria porque sólo se obtuvieron niveles de aglutinación bajos con las muestras infectadas y porque se obtuvieron resultados variables e inconsistentes con ambos clones, sanos e infectados. Sin embargo, ELISA dió consistentemente valores espectrofotométricos altos y reacciones intensas a simple vista para las muestras infectadas pero no para los clones sanos. Los resultados indican que ELISA puede usarse para detectar PVX y PVS en plántulas de cultivo de tejido y que en programas en los que se utiliza cultivo de tejidos es posible la detección temprana y eliminación de las plántulas infectadas.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Literature Cited
Banttari, E.E. and G.D. Franc. 1982. Enzyme-linked immunosorbent assay with single or combined antisera for viruses X and S in potato tubers and plants. Am Potato J 59:375–389.
Bokx, J.A. de (ed.). 1972. Viruses of potatoes and seed potato production. Wageningen, The Netherlands. 233 pp.
Casper, R. 1977. Detection of potato leafroll virus in potato andPhysalis floridana by enzyme-linked immunosorbent assay (ELISA). Phytopath Z 90:364–368.
Clark, M.F. and A.N. Adams. 1977. Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J Gen Virol 34:475–483.
Franc, G.D. and E.E. Banttari. 1981. Comparison of latex agglutination, radial diffusion, enzyme-linked immunosorbent assay, and indicator plants for the detection of potato virus S and potato virus X. Am Potato J 58:501. (Abstr.).
Gugleri, P. 1978. The detection of two potato viruses by enzyme-linked immunosorbent assay (ELISA). Phytopath Z 92:51–56.
Gutbrod, O. and J. Kelley. 1982. Rapid multiplication in seed certification programs. Am Potato J 59:469. (Abstr.).
Hoyman, W.G. 1964. Red Pontiac vine and tuber yields as affected by virus X. Am Potato J 21:278–283.
Jones, E.D. 1981. Production of potato nuclear seed stocks by tissue culture. Am Potato J 58:506–507. (Abstr.).
Jones, E.D., J. Munro and H.M. Darling. 1981. Seed potato certification.In: Hooker, W.J. (ed.). 1981 Compendium of Potato Diseases. The American Phytopathological Society, St. Paul, MN. 125 pp.
Khan, M.A. and S.A. Slack. 1978. Studies on the sensitivity of a latex agglutination test for the serological detection of potato virus X and potato virus S in Wisconsin. Am Potato J 55:627–637.
Khan, M.A. and S.A. Slack. 1980. Detection of potato viruses S and X in dormant potato tubers by the latex agglutination test. Am Potato J 57:213–218.
Lundsgaard, T. 1976. Routine seed health testing for barley stripe mosaic virus in barley seeds using the latex test. Z PlfanzenKr PlfanzenSchutz 83:278–283.
Maat, D.Z. 1978. Enzyme-linked immunosorbent assay (ELISA) for the detection of potato viruses A and Y in potato leaves and sprouts. Neth J Plant Pathol 84:167–178.
Manzer, F.E., D.C. Merriam and P.R. Hepler. 1978. Effects of potato virus S and two strains of potato virus X on yields of Russet Burbank, Kennebec and Katahdin cultivars in Maine. Am Potato J 55:601–609.
Manzer, F.E., D.C. Merriam and P.R. Hepler. 1979. Effects of potato virus S and mild potato virus X on early and late harvest yields of the Kennebec cultivar in Maine. Am Potato J 56:435–439.
Marcussen, O.F. and T. Lundsgaard. 1975. A new micromethod for the latex agglutination test. Z PlfanzenKr PlfanzenSchutz 82:547–548.
McLaughlin, M.R. and O.W. Barnett. 1979. The influence of plant sap and antigen buffer additives on the enzyme-immunoassay of two plant viruses. Phytopathology 69:1038. (Abstr.).
McMorran, J.P. and T.C. Allen. 1983. Maintenance, symptoms and distribution of potato viruses X, S, Y, A, M and leafroll in potato tissue culture plantlets. Am Potato J 60: 839–847.
Schultz, E.S. and R. Bonde. 1944. The effect of latent mosaic (virus X) on yield of potatoes in Maine. Am Potato J 21:278–283.
Slack, S.A. 1980. Pathogen-free plants by tissue culture. Plant Dis 64:14–17.
Wright, N.S. 1970. Combined effects of potato viruses X and S on yield of Netted Gem and White Rose potatoes. Am Potato J 47:475–478.
Wright, N.S. 1977. The effect of separate infections by potato viruses X and S on Netted Gem potato. Am Potato J 54:147–149.
Wright, N.S. and S.H. DeBoer. 1982. Seed potato pathogen control strategies in British Columbia. Am Potato J 59:491. (Abstr.).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gallenberg, D.J., Jones, E.D. Detection of potato viruses X and S in tissue culture plantlets. American Potato Journal 62, 111–118 (1985). https://doi.org/10.1007/BF02871339
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02871339