Summary
The exocrine and endocrine pancreas was investigated according to the fluorescence histochemical method of Falck and Hillarp. 1) Green fluorescent adrenergic fibers were regularly seen associated with arteries and arterioles in the exocrine pancreas. 2) Cholinergic fibers as shown by cholinesterase activity, were also found in the parenchyma of pancreas. 3) Yellow fluorescent cells scattered in the exocrine parenchyma and localized to a population of pancreatic islet cells with a characteristic distribution at the islet periphery were found. 4) By the fluorescence microscopic observation, inter-or intralobular pancreatic ducts, involving the zymogen granules, can also be seen after treatment with HCL vapor. 5) Yellow fluorescent cells, β-cells containing insulin, remained at the Islet periphery. At present, the above mentioned yellow fluorescent cells are identified as containing HPP (Human pancreatic polypeptide) according to the immunofluorescence technique.
With the use of the Falck and Hillarp histochemical technique ethionine induced pancreatitis in cats has been investigated. 1) After seven days of ethionine (5 mg/kg BW oral ad.) treatment, pancreas showed histochemical changes such as hemorrhage, fat necrosis, destruction of acinar cells and degranulation of zymogen from the parenchyma of pancreas. 2) Oral administration of ethionine for ten days induced severe degranulation, rupture of vessels, especially of veins and venules and later influenced arteries or arterioles. 3) Necrosis and fibrosis began to appear in the spaces between the cellular debris and marked pancreatic atrophy could be found. 4) The destruction of Islets of Langerhans can be found in the ethionine induced pancreatic parenchyma. On the other hand, an increased number of Islets of Langerhans was also observed in the site of lobule. 5) The presented finding may also suggest that the duration of administration of ethionine is more important factor than graded doses of ethionine in the production of ethionine induced pancreatitis in cats.
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Falck, B. and Hillarp, N.: Fluorescence of catechol amines and related compounds condensed with formaldehyde. J. Histochem. Cytochem., 10: 348, 1962.
Falck, B.: Observation on the possibilities of the cellular localization of monoamines by a fluorescence method. Acta. Physiol. Scand., 56. Suppl.: 1, 1962.
Falck, B. and Torp, A.: A fluorescence method for histochemical demonstration of noradrenaline in the adrenal medulla. Med. Exp., 5: 429, 1961.
Corrodi, H.: Fluoreszenz methoden zur histochemischen sicht barmachung von monoaminen. 1. identifizierung der fluoreszierenden produkte aus modellversuchen mit 6, 7-dimethoxyiso chinolinolinderivaten und formaldehyd. Helvetica. Chemica. Acta., 267: 2425, 1963.
Falck, B. and Owman, C.H.: A detailed methodological description of the fluorescence method for the cellular demonstration of biogenic monoamines. Acta. Univ. Lundensis, Sectio II., 7: 1, 1965.
Corrodi, H., Hillarp, N.-A. and Jonsson, G.: Fluorescence methods for the histochemical demonstration of monoamines. 3. Sodium borohydride reduction of the fluorescent compounds as a specificity test. J. Histochem. Cytochem., 12: 243, 1968.
Geyer, G.: Histochemische reduktion von aldehyden mit natrium borohydrid. Acta. Histochem., 15: 1, 1963.
Björklung, A., et al.: A method for differentiating dopamine from noradrenaline in tissue sections by microspectrofluorometry. J. Histochem. Cytochem., 16: 263, 1968.
Björklund, A., et al.: Histochemical demonstration of tryptamine properties of the formaledhydeinduced fluorophores of tryptamine and related indole compounds in models. Acta. Physiol. Scand., Suppl. 318: 1, 1968.
Grimelius, L.: A silver nitrat stain for α2-cells in human pancreatic islets. Acta. Soc. Med., 73: 243, 1968.
Solcia, E., et al.: Lead-Haematoxylin as a stain for endocrine cells: Significance of staining and comparison with other selective methods. Histochemie., 20: 116, 1969.
Scott, H.D.: Rapid staining of beta cell granules. Stain Technol., 72: 267, 1952.
Jenning, B.M.: Aldehyde-fuchsin staining applied to frozen section for demonstrating pituitary and pancreatic beta cells. J. Histochem. cytochem., 13: 328, 1965.
Singh, I.: A modification of the Masson-Hamperl method for staining of argentaffin cells. Anat. Anz. Bd., Suppl.: 81, 1964.
Mcmanus, J.F.A.: Methods of general utility for routine study of tissues, Harper & Row, Staining methods, p. 62, John Weatherhill, New York, 1960.
Karnovsky, M.J., et al.: A direct-coloring thiocholine method for cholinesterase. J. Histochem. Cytochem., 12: 219, 1964.
Alm, P., et al.: L-dopa turnover in the mouse pancreas. Life. Sciences., 6: 913, 1967.
Alm, P.: Fluorescence microscopy of the 5-HTP turnover in the exocrine pancreas of mice and rats. Z. Zellforsch., 96: 212, 1969.
Alm, P., et al.: Effects of pilocarpine on L-dopa turnover in the exocrine rat pancreas. Acta. Physiol. Scand., 83: 269, 1971.
Cegrell, L.: The occurrence of biogenic monoamines in the mammalian endocrine pancreas. Acta. Physiol. Scand., Suppl. 314: 1, 1968.
Cegrell, L., et al.: Catechol and indol derivation in a transplantable islet cell tumour of the golden hamster. Acta. Physiol. Scand., 77: 23, 1969.
Rich, A.R., et al.: Experimental and pathological studies on the pathogenesis of acute hemorrhagic pancreatitis. Bull. Johns Hopkins Hosp., 58: 212, 1936.
Frank, J., et al.: Some effects of ethionine in the dog with particular reference to external pancreatic secretion. Gastroenterology, 27: 743, 1954.
Alvin, M., et al.: Experimental hemorrhagic pancreatitis produced by staphylococcal toxin. Surgery., 47: 587, 1960.
Lester, F., et al.: Natural variation in experimental hemorrhagic pancreatitis. Surg. Gynecol. Obstet., 124: 531, 1967.
Farber, E. and Popper, H.: Production of acute pancreatitis with ethionine and its prevention by methionine. Proc. Soc. Exp. Biol. Med., 74: 838, 1950.
Goldberg, R.C., Chaikoff, I.L. and Dodge, A.H.: Destruction of pancreatic acinar tissue by dlethionine. Proc. Soc. Exp. Biol. Med., 74: 869, 1950.
De Almeida, A.L. and Grossman, M.I.: Experimental production of pancreatitis with ethionine. Gastroenterology., 20: 554, 1952.
Nishizaki, H.: The effect of alternation in dietary levels on pancreatic enzymes in rats. Folia. Endocrinol. Jpn., 35: 929, 1959.
Hamberger, B., et al.: Evidence for adrenergic nerve terminals and synapses in sympathetic ganglia. Int. J. Neuropharmacol., 2: 279, 1964.
Alm, P., et al.: Remarkable adrenergic nerves in the exocrine pancreas. Z. Zellforsch., 83: 178, 1967.
Spriggs, T.L.B., et al.: Controlled formaldehyde catecholamine condensation in cryostat sections to show adrenergic nerves by fluorescence. Stain Technology., 41: 323, 1966.
Fuxe, K., et al.: The distribution of adrenergic nerve fibers to the blood vessels in skeletal muscle. Acta Physiol. Scand., 64: 75, 1965.
Lever, J.D., et al.: Paravascular nervous distribution in the pancreas. J. Anat., 101: 189, 1967.
Falck, B., et al.: Evidence for the presence of biogenic amines in pancreatic islets. Experientia., 19: 139, 1963.
Håkansson, R., et al.: Elevated levels of insulin-like activity and 5-hydroxytryptamine in guinea pig pancreas following CoCl2 treatment. Endocrinology, 94: 324, 1974.
Larsson, L.-I., et al.: Localization of APP, a postulated new hormon, to a pancreatic endocrine cell type. Histochemistry., 42: 377, 1974.
Larsson, L.-I., et al.: Immunohistochemical localiization of human pancreatic polypeptide (HPP) to a population of islet cells. Cell Tissue Res., 156: 167, 1975.
Langslow, D.R.: Studies of the distribution of a new avian pancreatic polypeptide and insulin among bird, reptiles, amphibians and mammals. Endocrinology., 93: 558, 1973.
Braun-Blanquet, M.: Examen du pancréas de canard normal au microscope électronique précédé de son observation macroscopique et microscopique. 1. glande exocrine. Acta. Anat. (Basel). 72: 161, 1969.
Rich, A.R. and Duff, G.L.: Experimental and pathological studies on the pathogensis of acute hemorrhagic pancreatitis. Bull. Johns. Hopkins., 58: 212, 1936.
Grossman, M.I.: Experimental pancreatitis. Arch. Int. Med., 96: 298, 1955.
Kaiser, M.H. and Grossman, M.I.: Secretion of trypsin inhibitor in pancreatic juice. Gastroenteorlogy., 29: 35, 1955.
Kimmel, J.R., et al.: Isolation and characterization of chicken insulin. Endocrinology., 83: 1323, 1968.
Hazelwood, R.L., Turner, S.D., Kimmel, J.R. and Pollock, H.G.: Spectrum effects of a new polypeptide (third hormon?) isolated from the chicken pancreas. Gen. Comp. Endocrinol., 21: 485, 1973.
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Onda, M. Fluorescence histochemical study of the pancreas in the cat. Gastroenterol Jpn 11, 246–261 (1976). https://doi.org/10.1007/BF02777710
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DOI: https://doi.org/10.1007/BF02777710