Summary
Radioiodinated cell-surface antigens of Chang liver cells recognized with anti-liver-specific membrane lipoprotein (anti-LP-1) and anti-Tamm-Horsfall glycoprotein (anti-THGP) rabbit antibodies were analyzed by the immunoprecipitation followed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The analysis revealed that the antigens precipitated with anti-LP-1 were heterogeneous in molecular species consisting of 2 major and 5 minor polypeptides with molecular weights ranging from 36 × 103 (36 K) to 250 K daltons. That with anti-urinary THGP was a single polypeptide with a molecular weight of 67 K daltons, which was different from urinary THGP in molecular size, and was designated hepatic THGP.
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This research was supported in a part by a research grant from the Scientific Research Fund (1982, No. 57770490) of the Ministry of Education, Science and Culture of Japan.
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Sakaguchi, K., Koide, N., Kondow, H. et al. Hepatocyte plasma membrane antigens. II. Characterization of liver-specific membrane lipoprotein (LP-1) and tammhorsfall glycoprotein (THGP) like antigens (hepatic THGP) on the plasma membrane of chang liver cell. Gastroenterol Jpn 18, 339–345 (1983). https://doi.org/10.1007/BF02774951
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DOI: https://doi.org/10.1007/BF02774951