Summary
This study was designed to search for a way to inhibit pancreatic fibrosis following acute pancreatitis. Experimental necrotizing pancreatitis was induced by a freezing procedure in the pancreas of male Wistar rats. After the freezing procedure, the rats were divided into 3 groups: nothing addition was done to the control group, while the other 2 groups received daily intraperitoneal administration of antifibrotic substances (colchicine or L-azetidine-2-carboxylic acid (AZC)) for 6 weeks. Pancreatic enzymes in the serum were not markedly influenced by administration of antifibrotic substances, and there were no differences in the ratios of dry to wet weights of the pancreas between groups with and without these drugs. After freezing, the hydroxyproline levels in the pancreas of the control group increased from 1 to 4 weeks and then decreased during the 5th and 6th weeks. All groups receiving colchicine or AZC exhibited a significant decrease in the hydroxyproline levels at 2 to 4 weeks compared with the control group (P<0.01). Histological examination also showed the inhibition of pancreatic fibrosis, agreeing with changes in the hydroxyproline levels in groups receiving colchicine or AZC. These results suggest that administration of antifibrotic substances, colchicine and AZC, have the possibility of inhibiting pancreatic fibrosis following acute pancreatitis.
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References
Baggenstoss AH: Pathology of pancreatitis, in “Pancreatitis” edited by Gambill EE, CB Mosby Co, Saint Louis, 1973, p202
Sato T, et al: Chronic pancreatitis, in “Pancreatic Disease” edited by Yamagata K, Kanahara Co, Tokyo, 1976, p209(Jpn)
Rojkind M, et al: Colchicine and the treatment of liver cirrhosis. Lancet 6: 38, 1973
Kershenobich D, et al: Treatment of cirrhosis with colchicine, a double-blind randomized trial. Gastroenterology 77: 532, 1979
Snider GL: Interstitial pulmonary fibrosis which cell is the culprit? Am Rev Resp Dis 127: 535, 1983
Kinami Y, al.: Pancreatic fibrosis and collagenolytic activity following acute pancreatitis in the rat. J Kanazawa Med Univ 9: 187, 1984
Inayama S, et al: A new microanalytical method for determination of hydroxyproline in connective tissue. Keio J Med 27:43, 1978
Prockop DJ, et al: A specific method for the analysis of hydroxyproline in tissue and urine. Anal Biochem 1: 228, 1960
Neuman R, et al: The determination of hydroxyproline. J Biol Chem 184: 299, 1950
Diegelman R, et al: Inhibition of collagen secretion from bone and cultured fibroblasts by microtubular disruptive drugs. Proc Natl Acad Sci USA 69: 892, 1972
Ehrlich HP, et al: Microtubules in transcellular movement of procollagen. Nature New Biol 238: 257, 1972
Ehrlich HP, et al: Effects of antimicrotubular agents in the secretion of collagen. A biochemical and morphological study. J Cell Biol 62: 390, 1974
Rojkind M, et al: Effect of colchicine on collagen, albumine and transferrin synthesis by cirrhotic rat liver slices. Biochim Biophys Acta 378: 415, 1975
Takeuchi T, et al: Biosynthesis of abnormal collagens with amino acid analogues. I. Incorporation of Lazetidine-2-carboxylic acid and cis-4-fluoro-Lproline into protocollagen and collagen. Biochim Biophys Acta 175: 142, 1969
Takeuchi T, et al: Biosynthesis of abnormal collagens with amino acid analogus. II. Inability of cartilage cells to extrude collagen polypeptides containing Lazetidine-2-carboxylic acid or cis-4-fluoro-L-proline. Biochim Biophys Acta 175: 156, 1969
Uitto J, et al: Incorporation of proline analogues into polypeptides. Effects on the production of extracellular procollagen and on the stability of the triplehelical structure of the molecule. Biochim Biophys Acta 336: 234, 1974
Kershenobich D, et al: Effect of the administration of L-azetidine-2-carboxylic acid on albumin and transferrin biosynthesis by liver slices of rats treated with carbon tetrachloride. Biochim Biophys Acta 319: 216, 1973
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Kinami, Y., Sakata, N. & Takata, M. Effects of antifibrotic substances on pancreatic fibrosis following acute necrotizing pancreatitis. Gastroenterol Jpn 21, 499–507 (1986). https://doi.org/10.1007/BF02774634
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DOI: https://doi.org/10.1007/BF02774634