Abstract
A bacterial artificial chromosome (BAC) library of the 750-Mbp sugar beet genome represented in hybrid US H20 was constructed fromHind III-digested DNA, with an average insert size of 120 kbp. US H20 is a variety grown in the eastern United States. It exhibits heterosis for emergence and yield, presumably because of its hybridity between eastern and western US germplasm sources. Filter arrays were used to assess the abundance and distribution of particular nucleotide sequences. An rRNA gene probe found that 1.2% of the library carried sequences similar to these highly repetitive and conserved sequences. A simple sequence repeat element (CA)8 thought to be predominantly distributed throughout centromere regions of all chromosomes was present in 1.7% of clones. For more than half of the 28 randomly chosen expressed sequence tags (ESTs) used as probes, a higher-than-expected number of single-copy hybridization signals was observed. Assuming 6× genome coverage, this suggests that many duplicate genes exist in the beet genome.
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Abbreviations
- BAC:
-
bacterial artificial chromosome
- CMS:
-
cytoplasmic male sterility
- EST:
-
expressed sequence tag
- SNP:
-
single nucleotide polymorphism
References
Arumuganathan K and Earle ED (1991) Nuclear DNA content of some important plant species. Plant Mol Biol Rep 9: 208–18.
Clarke L and Carbon J (1976) A colony bank containing synthetic Col El hybrid plasmids representative of the entireE. coli genome. Cell 9: 91–100.
Coe GE and Hogaboam GJ (1971a) Registration of USH20 sugar beet. Crop Sci 11: 942.
Coe GE and Hogaboam GJ (1971b) Registration of sugarbeet parental line SP 6322-0. Crop Sci 11: 947.
de los Reyes BG, Myers SJ, and McGrath JM (2003) Differential stress-induction of glyoxylate cycle enzymes as a marker for seedling vigor in sugar beet (Beta vulgaris). Mol Gen Genomics 269: 692–8.
Doney DL (1995) USDA-ARS sugar beet releases. J Sugar Beet Res 32: 229–57.
Frijters ACJ, Zhang Z, van Damme M, Wang G-L, Ronald PC, and Michelmore RW (1997) Construction of a bacterial artificial chromosome library containing largeEcoRI andHindIII genomic fragments of lettuce. Theor Appl Genet 94: 390–9.
Gerlach WL and Bedbrook JR (1979) Cloning and characterization of ribosomal RNA genes from wheat and barley. Nucl Acids Res 7: 1869–85.
Gindullis F, Dechyeva D, and Schmidt T (2001) Construction and characterization of a BAC library for the molecular dissection of a single wild beet centromere and sugar beet (Beta vulgaris) genome analysis. Genome 44: 846–55.
Halldén C, Ahrén D, Hjerdin A, Säll T, and Nilsson N-O (1998) No conserved homoeologous regions found in the sugar beet genome. J Sugar Beet Res 35: 1–13.
Herwig R, Schulz B, Weisshaar B, Hennig S, Steinfath M, Drungowski M, Stahl D, Wruck W, Menze A, O'Brien J, and others (2002) Construction of a ‘unigene’ cDNA clone set by oligonucleotide fingerprinting allows access to 25,000 potential sugar beet genes. Plant J 32: 845–57.
Hogaboam GJ and Schneider CL (1982a) Registration of EL44 and EL44CMS sugarbeet parental lines. Crop Sci 22: 700.
Hogaboam GJ and Schneider CL (1982b) Registration of EL45/1 sugarbeet parental lines. Crop Sci 22: 700.
Hohmann U, Jacobs G, Telgmann A, Gaafar RM, Alam S, and Jung C (2003) A bacterial artificial chromosome (BAC) library of sugar beet and a physical map of the region encompassing the bolting gene B. Mol Genet Genomics 269: 126–36.
Luo MC, Thomas C, You FM, Hsiao J, Shu OY, Buell CR, Malandro M, McGuire PE, Anderson OD, and Dvorak J (2003) High-throughput fingerprinting of bacterial artificial chromosomes using the SNaPshot labeling kit and sizing of restriction fragments by capillary electrophoresis. Genomics 82: 378–89.
National Center for Biotechnology Information Home Page. Accessed 22 Mar. 2004 <http://www.ncbi.nlm.nih.gov>.
Owen FV (1945) Cytoplasmically inherited male-sterility in sugar beets. J Ag Res 71: 423–40.
Peters JL, Filip Cnudde F, and Gerats T (2003) Forward genetics and map-based cloning approaches. Trends Plant Sci 10: 484–91.
Schmidt T and Heslop-Harrison JS (1996) The physical and genomic organization of microsatellites in the sugar beet. Proc Nat Acad Sci USA 93: 8761–5.
Schneider K, Weisshaar B, Borchardt DC, and Salamini F (2001) SNP frequency and allelic haplotype structure ofBeta vulgaris expressed genes. Mol Breeding 8: 63–74.
Schnieder K, Schafer-Pregl R, Borchardt DC, and Salamini F (2002) Mapping QTLs for sucrose content, yield and quality in a sugar beet population fingerprinted by EST-related markers. Theor Appl Genet 104: 1107–13.
Schondelmaier J and Jung C (1997) Chromosomal assignment of the nine linkage groups of sugar beet (Beta vulgaris L.) using primary trisomics. Theor Appl Genet 95: 590–6.
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McGrath, J.M., Shaw, R.S., de los Reyes, B.G. et al. Construction of a sugar beet BAC library from a hybrid with diverse traits. Plant Mol Biol Rep 22, 23–28 (2004). https://doi.org/10.1007/BF02773345
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DOI: https://doi.org/10.1007/BF02773345