Abstract
Injections of octopamine, dopamine, and the octopaminergic agonists, clonidine and naphazoline, into the thoracic hemocoel enhanced female insemination in sugar-fed (sexually unreceptive)Phormia regina. Topical applications of the juvenile hormone (JH) analogue, methoprene, also enhanced female insemination in sugar-fed (sexually unreceptive)P. regina. Since JH plays a role in receptivity in protein-fed females, it was originally hypothesized that one agonist, clonidine, enhanced female insemination by acting on the corpus allatum (CA) to increase JH biosynthesis. Two or three doses of the antiallatal agent, precocene II, prior to administration of clonidine, did not inhibit clonidine-enhanced female insemination. Removal of the corpus allatum also did not have a significant effect on clonidine-enhanced female insemination. Measurement of juvenile hormone (JH) biosynthesis/release in corpora allata, which were removed at 1, 3, 5, and 7 h postinjection, revealed that clonidine does not affect JH biosynthesis/release. Our study reveals a possible role for the biogenic amines in female insemination in insects. We suggest that the octopaminergic agonist, clonidine, acts downstream from the corpus allatum on the regulatory mechanisms involved in female insemination.
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Adams, T. S., and Hintz, A. M. (1969). Relationship of age, ovarian development, and the corpus allatum to mating in the house-fly,Musca domestica.J. Insect Physiol. 15: 201–215.
Barton-Browne, L., Bartell, R. J., van Gerwen, A. C. M., and Lawrence, L. A. (1976). Relationship between protein ingestion and sexual receptivity in females of the Australian sheep blow flyLucilia cuprina.Physiol. Entomol. 1: 235–240.
Bouletreau-Merle, J, (1973). Receptivite sexuelle et vitellogenese chez femelles deDrosophila melanogaster: effect d’une application d’hormone juvenile et de deux analogues hormonaux.C.R. Acad. Sci. Paris 277: 2045–2048.
Brookhart, G. L., Edgecomb, R. S., and Murdock, L. L. (1987). Amphetamine and reserpine decrease biogenic amines and alter blow fly feeding behavior.J. Neurochem. 48: 1307–1315.
Chen, A. C., and Friedman, S. (1975). An isotonic saline for the adult blow fly,Phormia regina, and its application to perfusion experiments.J. Insect Physiol. 21: 529–536.
Christensen, T. A., Itagaki, H., Teal, P. E. A., Jasensky, R. D., Tumlinson, J. H., and Hildebrand, J. G. (1991). Innervation and neural regulation of the sex pheromone gland in femaleHeliothis moths.Proc. Natl. Acad. Sci. USA 88: 4971–4975.
Downer, G. H., and Hiripi, L. H. (1994). Biogenic amines in insects. In Borkovec, A. B., and Loeb, M. J. (eds.),Insect Neurochemistry and Neurophysiology, CRC Press, Boca Raton, FL, pp. 23–38.
Dudai, Y., and Zvi, S. (1984). [3H]Serotonin binds to two classes of sites inDrosophila head homogenate.Comp. Biochem. Physiol. 77C: 305–309.
Eldefrawi, M. E., Toppozada, A., Salpeter, M. M., and O’Brien, R. D. (1968). The location of penetration barriers in the ganglia of the American cockroachPeriplaneta americana (L.).J. Exp. Biol. 48: 325–338.
Evans, P. D. (1980). Biogenic amines in the insect nervous system.Adv. Insect Physiol. 15: 317–473.
Evans, P. D. (1981). Multiple receptor types for octopamine in the locust.J. Physiol. 318: 99–122.
Evans, A. M., and Green, K. L. (1990). The action of dopamine receptor antagonists on the secretory response of the cockroach salivary glandin vitro.Comp. Biochem. Physiol. 97C: 283–286.
Gillott, C., and Langley, P. A. (1981). The control of receptivity and ovulation in the tsetse flyGlossina morsitans.Physiol. Entomol. 6: 269–281.
Gwadz, R. W. (1972). Neuro-hormonal regulation of sexual receptivity in femaleAedes aegypti.J. Insect Physiol. 18: 259–266.
Hirashima, A., Tarui, H., Taniguchi, E., and Eto, M. (1994). Structure-activity studies of some putative octopaminergic agonists in the ventral nerve cord ofPeriplaneta americana, L.Pest.Biochem. Physiol. 50: 83–91.
Lane, N. J., and Swales, L. S. (1978). Changes in the blood-brain barrier of the central nervous system in the blow fly during development, with special reference to the formation and disaggregation of gap and tight junctions.Dev. Biol. 62: 415–431.
Lea, A. O. (1968). Mating without insemination in virginAedes aegypti.J. Insect Physiol. 14: 305–308.
Linn, C. E., Campbell, M. G., and Roelofs, W. L. (1992). Photoperiod cues and thermodulatory action of octopamine and 5-hydroxytryptamine on locomotor and pheromone response in male gypsy moths,Lymantria dispar. Arch. Insect Biochem. Physiol. 20: 265–284.
Linn, C. E., and Roelofs, W. L. (1986). Modulatory effects of octopamine and serotonin on male sensitivity and periodicity of response to sex pheromone in the cabbage looper moth,Trichoplusia ni. Arch. Insect Biochem. Physiol. 3: 161–171.
Liu, M.-A., Stoffolano, J. G., Jr., and Yin, C.-M. (1988). Conditions for estimation of corpus allatum activity in the blow flyPhormia regina, in vitro.Physiol. Entomol. 13: 69–79.
Manning, A. (1966). Corpus allatum and sexual receptivity in femaleDrosophila melanogaster.Nature 211: 1321–1322.
Milde, J. J., Ziegler, R., and Wallstein, M. (1995). Adipokinetic hormone stimulates neurones in the insect CNS.J. Exp. Biol. 198: 1307–1311.
Nagao, T., Tanimura, T., and Shimozawa, T. (1991). Neurohormonal control of the mating interval in the male cricket,Gryllus bimaculatus DeGeer.J. Comp. Physiol. A 168: 159–164.
Nijhout, H. F. (1977). Control of antennal hair erection in male mosquitoes.Biol. Bull. 153: 591–603.
Qin, W.-H. (1996).The Role of the Corpus Allatum in the Control of Life Processes in Phormia regina (Meigen), Master’s thesis, University of Massachusetts, Amherst.
Ringo, J. M., and Pratt, N. R. (1978). A juvenile hormone analogue induces precocious sexual behavior inDrosophila grimshawi (Diptera: Drosophilidae).Ann. Entomol. Soc. Am. 71: 264–266.
Roeder, T. (1994). Biogenic amines and their receptors in insects.Comp. Biochem. Physiol. 107C: 1–12.
Roelofs, W. L. (1995). Chemistry of sex attraction.Science 92: 44–49.
Sadou, F., Boschert, V., Amlaiky, N., Plassat, J. L., and Hen, R. (1992). A family ofDrosophila 5-HT receptors with distinct intracellular signalling properties and expression patterns.EMBO J. 11: 7–17.
Stoffolano, J. G., Jr. (1974). Influence of diapause and diet on the development of the gonads and the accessory reproductive glands of the black blow fly,Phormia regina (Meigen).Can. J. Zool. 52: 981–988.
Trabalon, M., and Campan, M. (1984). Etude de la receptivite sexuelle de la femelle deCalliphora vomitoria (Dipters, Calliphoridae) an cours du premier cycle gonadotrope I. Approches comportmentale et physiologuque.Behaviour 90: 241–258.
Yin, C.-M., and Stoffolano, J. G., Jr. (1990). The interactions among nutrition, endocrines, and and physiology on the reproductive development of the black blow fly,Phormia regina (Meigen).Bull. Inst. Zool. Acad. Sinica Monogr. 15: 87–108.
Yin, C.-M., Zou, B.-X., and Stoffolano, J. G., Jr. (1989. Precocene II treatment inhibits terminal oocyte development but not vitellogenin synthesis and release in the black blow fly,Phormia regina Meigen.J. Insect Physiol. 35: 465–474.
Yin, C.-M., Duan, H., and Stoffolano, J. G., Jr. (1993). Hormonal stimulation of the brain for its control of oögenesis inPhormia regina (Meigen).J. Insect Physiol. 39: 165–171.
Zou, B.-X., Yin, C.-M., Stoffolano, J. G., Jr., and Tobe, S. S. (1989). Juvenile hormone biosynthesis and release during oocyte development inPhormia regina Meigen.Physiol. Entomol. 14: 233–239.
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Evans, B.P., Stoffolano, J.G., Yin, CM. et al. A pharmacological and endocrinological study of female insemination inPhormia regina (Diptera: Calliphoridae). J Insect Behav 10, 493–508 (1997). https://doi.org/10.1007/BF02765373
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DOI: https://doi.org/10.1007/BF02765373