Abstract
The Acyl-CoA oxidase (AOX) isozymes catalyze the first steps of peroxisomal β-oxidation, which is important for the degradation of fatty acids. Using conserved blocks in previously identified yeastPOX genes encoding AOXs, the authors have shown that fivePOX genes are present in the yeastYarrowia lipolytica. These genes show approx 63% identity among themselves, and 42% identity with thePOX genes from other yeasts. Mono-disruptedY. lipolytica strains were constructed using a variation of the sticky-end polymerase chain reaction method. AOX activity in the mono-disrupted strains revealed that a long-chain oxidase is encoded by thePOX2 gene and a short-chain oxidase by thePOX3 gene.
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Wang, H., Le Dall, MT., Waché, Y. et al. Cloning, sequencing, and characterization of five genes coding for Acyl-CoA oxidase isozymes in the yeastYarrowia lipolytica . Cell Biochem Biophys 31, 165–174 (1999). https://doi.org/10.1007/BF02738170
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DOI: https://doi.org/10.1007/BF02738170