Abstract
A fuzzy control system was organized and applied to the control of ethanol concentration in a fed-batch cultivation process for emulsan production byAcinetobacter calcoaceticus RAG-1. The membership functions and fuzzy rules were determined by sets of data and experiences obtained from the preliminary culture experiments. The input variables, error (the difference between the set point value and the process variable) and the change of the error, were fuzzified by using the membership functions and the output variable, change of the ethanol feed rate, was inferred based on the membership functions and the given fuzzy rules. To obtain the numerical value for the output variable, the center-of-gravity method was used in the defuzzification procedure. The results showed that the ethanol concentration was well regulated around optimal level and the emulsan yield was increased compared with that of the cultivation controlled by the conventional feedback control loop.
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References
Abbott, B. J., “Ethanol Inhibition of a Bacterium(Acinetobacter calcoaceticus) in Chemostat Culture“,J. Gen. Microbiol,75, 383 (1973).
Abbott, B.J., Laskin, A.I. and McCoy, C.J., “Growth ofAcinetobacter calcoaceticus on Ethanol”,Appl. Microbiol,25, 787 (1973).
Alfafara, C. G., Miura, K., Shimizu, H., Shioya, S., Suga, K. and Suzuki, K., “Fuzzy Control of Ethanol Concentration and Its Application to Maximum Glutathione Production in Yeast Fed-Batch Culture”,Biotechnol. Bioeng.,41, 493 (1993).
Choi, J. W., Choi, H. G. and Lee, W. H., “Effects of Ethanol and Phosphate on Emulsan Production byAcinetobacter calcoaceticus RAG-1”,J. Biotechnol.,45, 217 (1996a).
Choi, J.W., Choi, H.G., Lee, S.B. and Lee, W.H., “Kinetic Model for Effects of Ethanol and Phosphate on Cell Growth and Emulsan Production inAcinetobacter calcoaceticus RAG-1”,Korean J. Chem. Eng.,13(3), 266 (1996b).
Choi, J.W., Choi, H.G., Lee, K.S. and Lee, W.H., “Control of Ethanol Concentration in a Fed-batch Cultivation ofAcinetobacter calcoaceticus RAG-1 using Feedback-assisted Iterative Learning Algorithm”,J. Biotechnol,49, 29 (1996c).
Czogala, E. and Rawlik, T., “Modelling of a Fuzzy Controller with Application to the Control of Biological Process”,Fuzzy Sets and Systems,31, 13 (1989).
Dohnal, M., “Fuzzy Bioengineering Models”,Biotechnol. Bioeng.,27, 1146 (1985).
Dubois, M., Gillies, K.A., Hamilton, J.K., Rebers, P.A. and Smith, F., “Calorimetric Method for Determination of Sugars and Related Substances”,Anal. Chem.,28, 350 (1959).
Filev, D. P., Kishimoto, M., Sengupta, S., Yoshida, Y. and Taguchi, H., “Application of the Fuzzy Theory to Simulation of Batch Fermentation”,J. Ferment. Technol,63(6), 545 (1985).
Gutnick, D. L. and Shabtai, Y., “Exopolysaccharide Bioemulsifier”, Biosurfactants and Biotechnology, Kosaric, N., Cairns, W. L. and Gray, N. C. C., eds., Marcel Dekker, Inc., New York, 1987.
Katayama, R., Kuwata, K., Kajitani, Y., Watanabe, M. and Nishda, Y., “Dimension Analysis of Chaotic Time Series Using Self-Generating Neuro Fuzzy Model”, The Proceeding of the 5th IFSA World Congress, 857 (1993).
Kishimoto, M., Kitta, Y., Takeuchi, S., Nakajima, M. and Yoshida, T., “Computer Control of Glutamic Acid Production Based on Fuzzy Clusterization of Culture Phases”,J. Ferment. Bioeng.,72(2), 110 (1991).
Konstantinov, K. B. and Yoshida, T., “Knowledge-Based Control of Fermentation Process”,Biotechnol Bioeng.,39, 479 (1992).
Postlethwaite, B. E., “A Fuzzy Estimator for Fed-Batch Fermentation”,Chem. Eng. Res. Des.,67, 267 (1989).
Reisfeld, A., Rosenberg, E. and Gutnick, D. L., “Microbial Degradation of Crude Oil: Factors Affecting the Dispersion in Sea Water by Mixed and Pure Culture”,Appl. Microbiol,24, 363 (1972).
Rosenberg, E., Zuckerberg, A., Rubinowitz, C. and Gutnick, D. L., “Emulsifier ofArthrobacter RAG-1: Isolation and Emulsifying Properties”,Appl. Environ. Microbiol,37, 402 (1979a).
Rosenberg, E., Perry, A., Gibson, D.T. and Gutnick, D.L., “Emulsifier ofArthrobacter RAG-1: Specificity Hydrocarbon Substrate”,Appl Environ. Microbiol,37, 409 (1979b).
Rosenberg, E., Zosim, Z., Belsky, I. and Gutnick, D. L., “Interaction ofAcinetobacter RAG-1 Emulsan with Hydrocarbon”, Proceedings of the Sixth International Fermentation Symposium, Advances in Biotechnology, vol. 3, Fermentation Product, Moo-Young, M., Robinson, C.W. and Vezina, C., eds., Pergamon Press, Toronto, 1980.
Schaefer, E., “Synthesis and Release of Emulsan byAcinetobacter calcoaceticus”, Ph.D. Thesis, Massachusetts Institute of Technology, Cambridge, MA, 1985.
Smith, C. A. and Corripio, A. B., “Principles and Practice of Automatic Process Control”, John Wiley & Sons, Inc., New York, 1985.
Stephanopoulos, G., “Artificial Intelligence in the Development and Design of Biochemical Processes”,Trends Biotechnol,4, 241 (1986).
Stephanopoulos, G., “The Future of Expert Systems in Chemical Engineering”,Chem. Eng. Prog.,83, 44 (1987).
Thibault, J., Breusegem, V. V. and Cheruy, A., “On-Line Prediction of Fermentation Variables Using Neural Networks”,Biotechnol. Bioeng.,36, 1041 (1990).
Wang, S. D. and Wang, D. I. C., “Cell Adsorption and Local Accumulation of Extracellular Polysaccharide in an ImmobilizedAcinetobacter calcoaceticus System”,Biotechnol. Bioeng.,34, 1261 (1989).
Yamakawa, T., “A Fuzzy Logic Controller”,J. Biotechnol,24, 1 (1992).
Zhang, B. S. and Edmunds, J. M., “Self-Organizing Fuzzy Logic Controller”,IEEE Proceedings-D,139(5), 460 (1992).
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Choi, JW., Oh, SM., Choi, HG. et al. Fuzzy control of ethanol concentration for emulsan production in a fed-batch cultivation ofAcinetobacter Calcoaceticus RAG-1. Korean J. Chem. Eng. 15, 310–316 (1998). https://doi.org/10.1007/BF02707086
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DOI: https://doi.org/10.1007/BF02707086