Abstract
Reversed-phase high-performance liquid chromatography was successfully developed for the simultaneous and rapid separation for the main whey proteins, α-Lactalbumin and β-Lactoglobulin. This method consisted of a linear gradient of the two mobile phases of 0.1% trifluoroacetic acid in water and 0.1% trifluoroacetic acid in acetonitrile. The total run time for this separation was approximately 30 min, and α-Lactalbumin was eluted followed byβ- Lactoglobulin. The injection volume was fixed at 20 μl and the flow rate was 1 ml 1/min. The optimum mobile phase composition and gradient conditions to separate α-Lactalbumin and β-Lactoglobulin (A+B) were experimentally obtained at the 15 μm particle with a pore size of 300 Å on the linear-gradient mode.
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Jung, Y.A., Choi, D.Y., Hong, S.B. et al. Mobile phase composition for resolving whey proteins in reversed-phase high performance liquid chromatography. Korean J. Chem. Eng. 20, 705–708 (2003). https://doi.org/10.1007/BF02706912
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DOI: https://doi.org/10.1007/BF02706912