Abstract
A mathematical model was formulated to describe growth and cloned protein production in the recombinantEscherichia coli cells containingphoA-directed expression systems. Kinetic parameters for the strains with two fusion genes (phoA- lacZ either on the chromosome or on a multicopy plasmid andphoA- amyE on a multicopy plasmid) were estimated and compared to analyze the effects of cloning site (chromosome and plasmid), product type(E. coli β-galactosidase andBacillus subtilis α-amylase), and culture temperature on the cell’s behavior. The presence of a multicopy plasmid reduced the specific growth rate and the phosphate uptake rate of the cell, both by 10%, compared with those of the chromosome-integrated strain. The overexpression ofB. subtilis a-amylase decreased the specific growth rate and the glucose consumption rate more than the β-galactosidase overproduction system. The presence of multiple copies of thephoA promoter on either an intactphoA gene or the fusion gene reduced both the repression and derepression efficiencies. Culture temperatures showed a significant effect on a-amylase production. A temperature of 30°C is more desirable than 37°C for α-amylase production in the recombinantE. coli containing thephoA promoter.
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Shin, P.K., Koo, J.H., Lee, W.J. et al. Modeling of cell growth andphoA-directed expression of cloned genes in recombinantEscherichia coli . Korean J. Chem. Eng. 13, 82–87 (1996). https://doi.org/10.1007/BF02705893
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DOI: https://doi.org/10.1007/BF02705893