Abstract
A recycling preparative HPLC was used to extract and separate the eupatilin contained inArtemisia princeps PAMPAN, and the optimum operating condition was experimentally determined. Eupatilin was extracted by ethanol solvent from the leaf and trunkof Artemisia princeps PAMPAN. The resulting solution was further partitioned with n-hexane, chloroform and ethyl acetate. The solution containing eupatilin was collected using a preparative as well as analytical column, and it was identified by LC-CE-MS. In the experiment, the mobile phase consisted of water/aceto-nitrile/TFA=50/50/0.5 (vol%), and UV wavelength measured was 370 nm. For analytical chromatography, the injection volume was 20 µL and the flow rate was fixed at 1.0 mL/min. The measured retention time for eupatilin was approximately 8.6 min in the above operating condition. For recycling preparative HPLC with commercially available GSA 310A column at 1.5 mL/min of the flow rate and 2 mL of injection volume, the purity of the eupatilin was almost 100% after recycling twice.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Ahn, B. O., Ryu, B. K., Ko, J. I., Oh, T. Y., Kim, S. H., Kim, W.B., Yang, J., Lee, E. B. and Hahm, K. B., “Beneficial effect of DA-9601, an extract of Artemisia herba, on animals models of inflammatory bowel disease,”J. Appl. Pharmacol.,5, 165 (1997).
Choi, Y J., Han, S. K., Chung, S. T. and Row, K. H., “Chromatographic separation of bupivacaine racemate by mathematical model with competitive Langmuir isotherm,”Korean J. Chem. Eng.,21, 829 (2004).
Hahm, K. B., Kim, J. H., You, B. M., Kim, Y S., Cho, S. W., Yim, H., Ahn, B. O. and Kim, W. B., “Induction of apoptosis with an extract of Artemisia asiatica attenuates the severity of cerulein-induced pancreatitis in rats,”Pancreas,17, 153 (1998).
Hong, S. R. and Row, K. H., “Separation of Acnthoside-Din Acanthopanax Senticosus by preparative recycling chromatography,”HWA-HAKKONGHAK,40, 488 (2002).
Kim, S. H., Kim, S. H., Lee, S. D., Kim, W. B., Lee, M. G. and Kim, N. D., “Determination of a new antiulcer agent, eupatilin, in rat plasma, bile, urine, and liver homogenate by high performance liquid chromatography,”Res. Comm. Mole. Path. andPharm.,97, 165 (1997).
Koshihara, Y., Neichi, T., Murota, S., Lao, A., Fujimoto, Y. and Tatsuno, T., “Selective inhibition of 5-lipoxygenase by natural compounds isolated from Chinese plants, Artemisia rubripes Nakai,”FEBS Letters,158, 41 (1983).
Lee, S. J., “Studies on the identification of Korean traditional folk medicine,”Kor. J. RawMed.,6, 75 (1975).
Lee, S. J., Oh, T. Y., Ahn, B. O., Cho, H., Kim, W. B., Kim, Y B., Surh, Y J. and Hahm, K. B., “Involvement of oxidative stress in experimentally induced reflux esophagitis and Barrett’sesophagus: clue for chemoprotection by antioxidant treatment,”Mutat. Res., in press.
Lee, Y W., So, M. S., Lee, J. W., Chung, S. T. and Row, K. H., “Retention models of capacity factor with different compositions of organic modifier in RP-HPLC,”Korean J. Chem. Eng.,13, 578 (1996).
Oh, T. Y., Ryu, B. K., Ko, J. I., Ahn, B. O., Kim, S. H., Kim, W. B., Lee, E. B., Jin, J. H. and Hahm, K. B., “Protective effect of DA-9601, an extract of Artemisia herba, against naproxen-induced gastric damage in arthritis rats,”Pharmcol. Res.,20, 414 (1997).
Seo, H. J., Park, K. K. and Surh, Y J., “Suppression of mouse skin tumor promotion and induction of apoptosis in HL-60 cells by Artemisia asiatica (Asteraceae),”Programs and Abstracts of the 2 nd International Conference on Food Factors,161, 12 (1999).
Seo, H. J. and Surh, Y.J., “Eupatilin, a pharmacologically active flavone derived from Artemisa plants, induces apoptosis in human promyelocytic leukemia cells,”MutationRes.,496, 191 (2001).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Lee, YW., Jin, Y. & Row, K.H. Extraction and purification of eupatilin fromArtemisia princeps PAMPAN recycling preparative HPLC. Korean J. Chem. Eng. 23, 279–282 (2006). https://doi.org/10.1007/BF02705727
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02705727