Abstract
A D-phenylalanine (D-Phe) imprinted terpolymer P(AN-AA-AAm) membrane was prepared by the wet phase inversion method. Acrylamide (AAm) and acrylic acid (AA) were used as the functional monomer and acrylonitrile (AN) was used as a physical cross linker. The template molecules were removed from the terpolymer matrix by washing with a 5 percent acetic acid solution. The removal of template molecules from the membrane matrix increased the population of free COOH groups and reduced that of dimerized COOH groups in the membrane matrix, which is an indirect evidence of the formation of recognition sites. The adsorption selectivity of the D-Phe imprinted terpolymer membrane prepared by in-situ implanting method was 0.37 at pH 2 after 3 h batch adsorption using 100 mg Phe/l racemate solution and reached nearly 1 after 24 h. In the ultrafiltration process, the permselectivity was 0.38 at pH 2 after 2.5 min. Separation of D-Phe from the racemate solution was demonstrated by a repeated ultrafiltration batch work.
Article PDF
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
References
Araki, K., Goto, M. and Furusaki, S., “Enantioselective Polymer Prepared by Surface Imprinting Technique Using a Bifunctional Molecule,”Anal. Chim. Acta.,469, 173 (2002).
Chen, Y., Kele, M., Quinones, I., Sellergren, B. and Guiochon, G., “Influence of the pH on the Behavior of an Imprinted Polymeric Stationary Phase-Supporting Evidence for a Binding Site Model,”J. Chromatogr. A,927, 1 (2001).
Mayes, A. G. and Mosbach, K., “Molecularly Imprinted Polymer Beads: Suspension Polymerization Using a Liquid Perfluorocarbon as the Dispersing Phase,”Anal. Chem.,68(21), 3769 (1996).
Jin, J. S., “Preparation of HPLC. Chiral Stationary Phases and Resolution of Enantiomers,” PhD Thesis, Pusan National University, Korea (1999)
Ohkubo, K., Urata, Y., Hirota, S., Honda, Y. and Sagawa, T., “Homogenous and Heterogenous Esterolytic Catalyses of Imidazole-containing Polymers Prepared by Molecular Imprinting of a Transition-state Analogue,”J. Mol. Catal.,87, L21 (1994).
Park, J. K. and Seo, J. I., “Characteristics of Phenylalanine Imprinted Membrane Prepared by the Wet Phase Inversion Method,”Korean J. Chem. Eng.,19, 940 (2002).
Park, J. K., Kim, S. J. and Lee, J. W., “Adsorption Selectivity of Phenylalanine Imprinted Polymer Prepared by the Wet Phase Inversion Method,”Korean J. Chem. Eng.,20, 1066 (2003).
Robinson, D. K. and Mosbach, K., “Imprinting of a Transition State Analogue Leads to a Polymer Exhibiting Esterolytic Activity”,J. Chem. Soc., Chem. Commun.,14, 969 (1989).
Wang, D., Hong, S. P., Yang, G. and Row, K. H., “Caffeine Molecular Imprinted Microgel Spheres by Precipitation Polymerization,”Korean J. Chem. Eng.,20, 1073 (2003).
Wang, H. Y., Kobayashi, T. and Fujii, N., “Molecular Imprint Membranes Prepared by the Phase Inversion Precipitation Technique,”Langmuir,12, 4850 (1996).
Wang, H. Y., Kobayashi, T., Fukaya, T. and Fujii, N., “Molecular Imprint Membranes Prepared by the Phase Inversion Technique. 2. Influence of Coagulation Temperature in the Phase Inversion Process on the Encoding in Polymeric Membranes,”Langmuir,13, 5396 (1997).
Whitcombe, M. J., Rodriquez, M. E., Villar, P. and Vulfson, E. N., “A New Method for the Introduction of Recognition Site Functionality into Polymers Prepared by Molecular Imprinting: Synthesis and Characterization of Polymeric Receptors for Cholesterol,”J. Am. Chem. Soc.,117, 7105 (1995).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Park, J.K., Kim, S.J. Separation of phenylalanine by ultrafiltration using D-Phe imprinted polyacrylonitrile-poly(acrylic acid)-poly(acryl amide) terpolymer membrane. Korean J. Chem. Eng. 21, 994–998 (2004). https://doi.org/10.1007/BF02705583
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02705583