Abstract
Three strong anion-exchange membranes (CIM QA, Q100 and HiTrap Q) were investigated for the separation of the major proteins, which were contained in whey, such as α-Lactalbumin, BSA and β-Lactoglobulin. Experiments were performed to determine the optimum mobile phase composition for separating the whey proteins using the standard chemicals of the proteins. The mobile phase was buffer A (20 mM piperazine-HCl pH 6.4) and buffer B (buffer A+1 M NaCl) and the linear gradient elution changes of salt concentration were applied. The standard chemicals of the proteins were used to investigate the optimal mobile phase compositions with the three anion-exchange membranes. From the experimental results, it was found that HiTrap Q was the most effective in separating whey proteins.
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Kim, J.I., Choi, D.Y. & Row, K.H. Separation of whey proteins by anion-exchange membranes. Korean J. Chem. Eng. 20, 538–541 (2003). https://doi.org/10.1007/BF02705562
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DOI: https://doi.org/10.1007/BF02705562