Abstract
Reactive aqueous two-phase partition of cellulase by modification with amphiphilic copolymer was studied. The modifier of cellulase could be directly used as a polymer of two-phase system. The maleic acid anhydride functional group of the synthetic copolymer covalently coupled with the amino groups of cellulase molecule. The degree of modification of the amino groups was controlled by the types of synthetic copolymer or the weight ratio of a synthetic copolymer to cellulase. As the degree of modification of cellulase increased, the modified cellulase moved to the copolymer phase of the copolymer/dextran two-phase system.
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Abbreviations
- EO/AO:
-
ethylene oxide concentration in PAG chain [%]
- DM:
-
degree of modification [%]
- k:
-
the number of copolymerization
- K:
-
the value of partition coefficient
- R:
-
recovery rate [%]
- (AO):
-
the total of EO and PO in PAG chain
- CP:
-
cloudy point [°C ]
References
Habeeb, A. F. S. F.:Anal. Biochem.,14, 328(1966).
Hartree, M. M., Hogan, C. M. and Saddler, J. N.:Biotechnol. Bioeng.,30, 558(1987).
Kajiuchi, T. and Park, J.W.:J. Client. Eng. Japan. 25, 202 (1992).
Kajiuchi, T. and Park. J. W.: Proc. of APBIoCHEC ’92, 106 (1992).
Mandels, M., Andreotti, R. and Roche, C:Biotechnol Bioeng. Symp.,6, 21(1976).
Miller, G. L.:Anal. Chem.,31. 426(1959).
Kajiuchi, T.. Park, J.W. and Moon, H. Y.:J. Chem. Eng. Japan,26, 28 (1993).
Park, J. W., Lee, S. M. and Kajiuchi, T.:J. Chem. Eng. Japan. 23, 497(1990).
Moloney, A. and Coughlan, M. P.:Biotechnol. Bioeng.,25, 271 (1983).
Ooshina, H., Sakata, M. and Harano, Y.:Biotechnol. Bioeng.,28, 1727(1986).
Fields, R.:Biochem. J.,124, 581(1971).
Beldman, G., Van Leeuwen, M. F. S., Rombauts, F. M. and Voragen, F. G. J.:Eur. J. Biochem.,146, 302 (1985).
Shikata, S. and Nishizawa,K.J. Biochem.,78, 499 (1978).
Tanaka, M. et al.:Appl. Microbiol. Biotechnol.,23, 263 (1986).
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Moon, HY., Kim, SY., Park, JW. et al. Reactive aqueous two-phase partition of cellulase. Korean J. Chem. Eng. 10, 235–239 (1993). https://doi.org/10.1007/BF02705273
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DOI: https://doi.org/10.1007/BF02705273