Abstract
The use of suspended and alginate-entrapped XAD-7, polycarboxyl ester resin, for thein situ separation of berberine, isoquinoline alkaloid, produced from plant cell culture ofThalictrum rugosum was investigated. XAD-7 could adsorb the berberine and the amount of berberine adsorbed on XAD-7 depended on pH. The neutral form of berberine was adsorbed onto XAD-7 and the adsorption isotherm for berberine showed a Langmuir-type appearance.In situ berberine removal enhanced the production of secondary metabolites in cell suspension culture ofThalictrum rugosum. Addition of XAD-7 at the exponential phase of cell growth was the most effective for enhancement of berberine production. In chitosan-treated cell culture to permeabilize intracellular berberine, berberine secretion was significantly accelerated by addition of alginate-entrapped XAD-7 at the stationary phase of cell growth and thus more than 70% of the produced berberine could be adsorbed to alginate-entrapped XAD-7.
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Abbreviations
- As :
-
amount of the adsorbent [g]
- C:
-
berberine concentration [mg/mL]
- C a o :
-
adsorbed berberine concentration [mg/mL]
- Co :
-
initial berberine concentration [mg/mL]
- C+ :
-
concentration of protonated forms of berberine [mg/mL]
- Co :
-
concentration of unprotonated forms of berberine [mg/mL]
- Q:
-
resin loading capacity [mg adsorbed/g adsorbent]
- VB :
-
liquid volume of berberine solution [mL]
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Choi, JW. In situ berberine separation with immobilized adsorbent in cell suspension cultures ofThalictrum rugosum . Korean J. Chem. Eng. 9, 128–134 (1992). https://doi.org/10.1007/BF02705129
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DOI: https://doi.org/10.1007/BF02705129