Abstract
Tyrosine phosphorylation events are key components of several cellular signal transduction pathways. This study describes a novel method for identification of substrates for tyrosine kinases. Co-expression of the tyrosine kinase EphB1 with the intracellular domain of guanylyl cyclase C (GCC) inEscherichia coli cells resulted in tyrosine phosphorylation of GCC, indicating that GCC is a potential substrate for tyrosine kinases. Indeed, GCC expressed in mammalian cells is tyrosine phosphorylated, suggesting that tyrosine phosphorylation may play a role in regulation of GCC signalling. This is the first demonstration of tyrosine phosphorylation of any member of the family of membrane-associated guanylyl cyclases.
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Abbreviations
- GCC:
-
Guanylyl cyclase C
- CFTR:
-
cystic fibrosis transmembrane regulator
- GST:
-
glutathione S transferase
- PKLD:
-
protein kinase-like domain
- CTD:
-
C-terminal domain
- GCD:
-
guanylyl cyclase domain
- PVDF:
-
polyvinylidene difluoride
- GCA:
-
guanylyl cyclase A
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Bhandari, R., Mathew, R., Vijayachandra, K. et al. Tyrosine phosphorylation of the human guanylyl cyclase C receptor. J Biosci 25, 339–346 (2000). https://doi.org/10.1007/BF02703787
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DOI: https://doi.org/10.1007/BF02703787