Abstract
The classical Ogston-Laurent gel model of size exclusion chromalography was modified to include both dextran fibers and cross-links. The dimensions of Sephadex gel fibers were determined from the experimental data in the literature and the microstructural study o’ Sephadex gel. The corresponding radii of the dextran chain and the cross-linking were calculated to be 7.8 x l(10*-8and 4.8 x 10*-8 cm, respectively. The lengths of the dextran chain varies from 1.45 x 1012 (G-200) lo 9.46 x 1012 cm/m/(G-25) while the length of the cross-link varies from 0.14 x 1012 (G-200) to 3.18 x 1012 cm/m/ (G-25). The partial specific volume of swollen Sephadex gel material was calculated to be 0.586 m//g.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Ogston, A.G.:Trans. Faraday Soc.,54, 1754 (1958).
Koo, Y.M.: Ph. D. Dissertation, Purdue University, West Lafayette, Indiana, U.S.A. (1985).
Koo, Y.M. and Wankat, P.C.:Korean Biochem. J.,21, 22(1988).
Laurent, T.C. and Killander, J.:J. Chromatogr.,14, 317(1964).
Koo, Y.M.:Korean J. of Biotech. and Bioeng.,4(1), 15 (1989).
Granath, K., in James, A.T., and Morris, L.J. (Editors): “New Biochemical Separations”, D. Van Nostrand, London, (1964).
Determann, H.: Gel Chromatography, Springer-Verlag, New York, NY (1968).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Koo, YM. Estimation of fiber radii and lengths of sephadex gel by the modified Ogston model. Korean J. Chem. Eng. 6, 254–258 (1989). https://doi.org/10.1007/BF02697688
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02697688