Abstract
A self-organizing fuzzy controller is constructed for control of substrate concentration in fed-batch operation of a cell culture process. A genetic algorithm is used to generate fuzzy rules of the self-organizing fuzzy controller and to modify the universe of discourse automatically. The fuzzy controller is designed for the application ofScutellaria baicalensis G. plant cell culture process as a model system. A substrate feeding strategy of a two stage culture method to maximize flavone glycoside production in fed-batch culture ofScutellaria baicalensis G. plant cell is proposed based on structured model of growth and product synthesis. As a two stage culture, the feeding strategy consists of the first period at 22 g/L of glucose concentration to promote cell growth and the second period at 10 g/L of glucose concentration to promote flavone glycoside synthesis. The designed self-organizing fuzzy controller is applied to regulate the glucose concentration at a given set-point to increase flavone glycoside synthesis. The simulation results show that the proposed feeding strategy in a fed-batch culture enhances flavone glycoside production and the self-organizing fuzzy logic controller generated by genetic algorithm improves controller performance.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Ahn, B.Z., Lee, Y.H., You, K.H. and Kim, S.Y., “Augmentation of the Cytotoxic Effects of Anticancer Drugs by (±)-ar-Tumerone and Extracts of the Lithosperma andScutellaria Roots against Leukemia Cell Lines”,Yakhak Hoeji,35, 203 (1991).
Buitelaar, R. M. and Tramper, J. M., “Strategies to Improve the Production of Secondary Metabolites with Plant Cell Cultures”,J. Biotechnol,23, 111 (1992).
Choi, J. W., Park, J. K., Lee, K. S. and Lee, W. H., “Application of Genetic Algorithm to Fuzzy Rule Generation in Fed-Batch Culture ofThalictrum rugosum Plant Cell”, Proceedings of the Asian Control Conference, 2, 305 (1994).
Choi, J.W., Choi, H.G., Oh, S.M., Lee, S.B., Lee, K.S. and Lee, W. H., “Fuzzy Control of Ethanol Concentration for Emulsan Production in a Fed-Batch Cultivation ofAcinetobacter Calcoaceticus RAG-1”, KoreanJ. Chem. Eng.,15, 310 (1998a).
Choi, J. W., Cho, J. M, Lee, J. G., Kim, I. H. and Park, Y. H., “A Structured Kinetic Model ofScutellaria baicalensis G. Plant Cell Cuture Process”,Korean Journal of Biotechnology and Bioengineering,13, 251 (1998b).
Dejong, K., “Learning with Genetic Algorithm”,Machine Learning,3, 121 (1988).
Hideo, T., Hisao, I., Ken, N. and Naohisa, Y., “Genetic Operations for Rule Selection in Fuzzy Classification Systems”, Proc. of Fifth IFSA World Congress,15 (1993).
Hwang, H. S. and Woo, K. B., “Automatic Fuzzy Rule Generation Utilizing Genetic Algorithms”,J. of Fuzzy Logic and Intelligent Systems,2, 40 (1989).
Lee, C. H., Jung, J. H. and Lee, I. B., “Scheduling of Multiproduct Batch Processes with Earliness and Trading Penalties Using Genetic Algorithm”,HWAHAK-KONGHAK,33, 734 (1995).
Matsubara, K., Kitani, S., Yoshioka, T., Morimoto, T. and Fujita, Y., “High Density Culture ofCoptis japonica Cells Increases Berberine Production”,J. Chem. Tech. Biotechnol.,46, 61 (1989).
Metzler, C. M., Elfring, G. L. and McWwen, A. J., “A Package of Computer Programs for Pharmacokinetic Modeling”,Biometrics,30, 562 (1974).
Postlethwaite, B.E., “A Fuzzy State Estimator for Fed-Batch Fermentation”,Chem. Eng. Res. Des.,67, 267 (1987).
Prenosil, J. and Pedersen, H., “Immobilized Plant Cell Reactors”,Enzyme Microb. Technol., 5, 323 (1983).
Seo, W. H., Choe, T. B. and Park, Y. H., “Identification and Production of Flavonoids in a Cell Suspension Culture ofScutellaria baicalensis G.”,Plant Cell Reports,12, 414 (1993).
Sugeno, M. and Nishida, M., “Fuzzy Control of Model Car.”,Fuzzy Set and Systems,16, 103 (1985).
Zadeh, L. A. and Lotfi, A., “Outline of a New Approach to the Analysis of Complex Systems and Decision Processes”,IEEE Tr. on Systems, Man, and Cybernetics,SMC-3, 28 (1993).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Choi, JW., Cho, J.M., Lee, J.G. et al. Application of genetic algorithm to self-organizing fuzzy controller in fed-batch culture ofScutellaria baicalensis G. plant cell. Korean J. Chem. Eng. 15, 404–410 (1998). https://doi.org/10.1007/BF02697130
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02697130