Abstract
The techniques of microdissection and microcloning have been applied to the isolation of B-chromosome DNA from rye. We have identified a DNA sequence on the rye B-chromosome which is homologous to an A-chromosome sequence, and which is dispersed and moderately repeated on the A- and B-chromosomes. This demonstrates that the rye B-chromosome is heterogeneous in the nature of its DNA sequence composition, containing sequences which are present on the A-chromosomes in addition to those not present on the A-chromosomes.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- UV:
-
ultraviolet light
- PEG:
-
polyethylene glycol
- DEAE:
-
diethylaminoethyl
- PCR:
-
polymerase chain reaction
References
Amos, A. and G. A. Dover. 1981. The distribution of repetitive DNAs between regular and supernumerary chromosomes in species ofGlossina (Tsetse): a two step process in the origin of supernumeraries. Chromosoma 81:673–690.
Bates, G. P., B. J. Wainwright, R. Williamson, and S. D. M. Brown. 1986. Microdissection of and microcloning from the short arm of human chromosomes 2. Mol. Cell. Biol. 6:3826–3830.
Brown, S. D. M. and A. J. Greenfield. 1987. A model to describe the size distribution of mammalian genomic fragments recovered by microcloning. Gene 55:327–332.
Davis, L. G., M. D. Dibner, and J. F. Battey. 1986. Growth and preparation of bacteriophage. InBasic Methods in Molecular Biology (Davis, L. G., Dibner, M. D. and Battey, J. F. eds.) pp. 158–160. Elsevier.
Dover, G. A. 1975. The heterogeneity of B-chromosome DNA: no evidence for a B-chromosome specific repetitive DNA correlated with B-chromosome effects on meiotic pairing inTriticinae. Chromosome 53:153–173.
Dover, G. A.. 1976. Molecular properties of B-chromosome DNA: a clue to its origin in theTriticinae andOrthoptera.Current Chromosome Research. Jones, K. and Brandham, P. E. (eds.) Elsevier/North-Holland, Biomedical Press, Amsterdam.
Feinberg, A. P. and B. Vogelstein. 1984. A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 137:266–267.
Fisher, E. M. C., J. S. Cavanna, and S. D. M. Brown. 1985. Microdissection and microcloning of the mouse X-chromosome. PNAS 82:5846–5849.
Jones, R. N. and H. R. Rees. 1982.B-Chromosomes. pp. 1–266. Academic Press.
Kishikawa, H. 1965. Cytogenetic studies of B-chromosomes in rye,Secale cereale L. in Japan. Agric. Bull. Saga Univ. 21:1–81.
Raeder, U. and P. Broda. 1985. Rapid preparation of DNA from filamentous fungi. Let. Appl. Microbiol. 1:17–21.
Reed, K. C. 1986a. Alkaline southern blotting to Zetaprobe membrane. Biorad Bulletin No. 1233.
Reed, K. C. 1986b. Nucleic acid hybridizations with DNA bound to Zetaprobe membrane. Biorad Bulletin No. 1234.
Rimpau, J. and R. B. Flavell. 1975. Characterization of rye B-chromosome DNA by DNA/DNA hybridization. Chromosoma 52:207–217.
Sandery, M. J., J. W. Forster, and R. N. Jones. 1989. Identification of a putative B-chromosome specific sequence from rye (Secale cereale). Heredity 62:282.
Scalenghe, F., E. Tusco, J. E. Edstrom, V. Pirotta, and M. L. Melli. 1981. Microdissection and cloning of DNA from a specific region ofDrosophila melanogaster polytene chromosomes. Chromosoma 82: 205–216.
Timmis, J. N., J. Ingle, J. Sinclair, and R. N. Jones. 1975. The genomic quality of rye B-chromosomes. J. Exp. Bot. 26:367–378.
Young, R. A. and R. W. Davis. 1983. Efficient isolation of genes using antibody probes. PNAS 80:1194–1198.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sandery, M.J., Forster, J.W., Macadam, S.R. et al. Isolation of a sequence common to A- and B-chromosomes of rye (Secale cereale) by microcloning. Plant Mol Biol Rep 9, 21–30 (1991). https://doi.org/10.1007/BF02669286
Issue Date:
DOI: https://doi.org/10.1007/BF02669286