Summary
Cultured cells from the bovine endosalpinx were used to evaluate effects of estradiol-17β, progesterone, epidermal growth factor, and insulinlike growth factors I and II on [3H]thymidine incorporation. Cells were treated with hormones and growth factors when approximately 50% confluent. After 24 h, DNA synthesis was quantified by pulsing cells with [3H]thymidine for 12 h and determining uptake into DNA. Cells prepared by mechanical dispersal incorporated more [3H]thymidine than cells dispersed with collagenase. However, hormonal responses were the same for both types of cells. As compared to plastic, cells on a Matrigel substratum exhibited lower incorporation of [3H]thymidine and were unresponsive to hormones. Estradiol-17β increased [3H]thymidine incorporation slightly at 10−10 mol/liter and higher. Epidermal growth factor, insulinlike growth factor-I, and insulinlike growth factor-II also stimulated [3H]thymidine incorporation. Effects of insulinlike growth factor-I were greater for cells treated with estradiol-17β. In the absence of estradiol, progesterone inhibited [3H]thymidine incorporation at 1, 10, and 100 ng/ml. When estradiol-17β was present, progesterone stimulated [3H]thymidine incorporation at 1 ng/ml and reduced incorporation at 100 ng/ml. In conclusion, [3H]thymidine incorporation by cultured oviductal endosalpingeal cells can be regulated by ovarian steroids and growth factors. These molecules may represent signals through which the ovary, embryo, and oviduct regulate oviductal growth.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Abe, H.; Oikawa, T. Effects of estrogen and progesterone on the cyto-differentiation of epithelial cells in the oviduct of the newborn Golden hamster. Anat. Rec. 235:390–398; 1993.
Boice, M. L.; Mavrogianis, P. A.; Murphy, C. N., et al. Immunocytochemical analysis of the association of bovine oviduct-specific glycoproteins with early embryos. J. Exp. Zool. 263:225–229; 1992.
Brenner, R. M.; West, N. B.; McClellan, M. C. Estrogen and progesterone receptors in the reproductive tract of male and female primates. Biol. Reprod. 42:11–19; 1990.
Brigstock, D. R. Growth factors in the uterus: Steroidal regulation and biological actions. Baillière’s Clin. Endocrinol. Metab. 5:791–806; 1991.
Buhi, W. C.; Ashworth, C. J.; Bazer, F. W., et al. In vitro synthesis of oviductal secretory proteins by estrogen-treated ovariectomized gilts. J. Exp. Zool. 262:426–435; 1992.
Chian, R.-I.; Sirard, M.-A. Fertilizing ability of bovine spermatozoa co-cultured with oviduct epithelial cells. Biol. Reprod. 52:156–162; 1995.
Dardik, A.; Smith, R. M.; Schultz, R. M. Colocalization of transforming growth factor-α and a functional epidermal growth factor receptor (EGFR) to the inner cell mass and preferential localization of the EGFR on the basolateral surface of the trophectoderm in the mouse blastocyst. Dev. Biol. 154:396–409; 1992.
de la Sota, R. L.; Lucy, M. C.; Staples, C. R., et al. Effects of recombinant bovine somatotropin (sometribove) on ovarian function in lactating and nonlactating dairy cows. J. Dairy Sci. 76:1002–1013; 1992.
Eyestone, W. H.; First, N. L. Co-culture of early cattle embryos to the blastocyst stage with oviductal tissue or in conditioned media. J. Reprod. Fertil. 85:715–720; 1989.
Ghahary, A.; Murphy, L. J. Uterine insulin-like growth factor-I receptors: Regulation by estrogen and variation throughout the estrous cycle. Endocrinology 125:597–604; 1989.
Giudice, L. C.; Dsupin, B. A.; Irwin, J. C., et al. Identification of insulin-like growth factor binding proteins in human oviduct. Fertil. Steril. 57:294–301; 1992.
Hisinuma, M.; Takahashi, Y.; Yanagawa, H. Isolation and monolayer culture of bovine oviduct epithelial cells. Jpn. J. Vet. Sci. 51:2101–2108; 1989.
Ignar-Trowbridge, D. M.; Nelson, K. G.; Bidwell, M. C., et al. Coupling of dual signaling pathways: Epidermal growth factor action involves the estrogen receptor. Proc. Natl. Acad. Sci. USA 89:4658–4662; 1992.
Ignar-Trowbridge, D. M.; Teng, C. T.; Ross, K. A., et al. Peptide growth factors elicit estrogen receptor-dependent transcriptional activation of an estrogen-responsive element. Mol. Endocrinol. 7:992–998; 1993.
Inaba, T.; Wiest, W. G.; Strickler, R. C., et al. Augmentation of the response of mouse uterine epithelial cells to estradiol by uterine stroma. Endocrinology 123:1253–1258; 1988.
Joshi, M. S. Growth and differentiation of the cultured secretory cells of the cow oviduct on reconstituted basement membrane. J. Exp. Zool. 260:229–238; 1991.
Kamwanja, L. A.; Hansen, P. J. Regulation of proliferation of bovine oviductal epithelial cells by estradiol: Interactions with progesterone, interferon-τ and interferon-α Horm. Metab. Res. 25:500–502; 1993.
Lei, Z. M.; Rao, C. V. Expression of epidermal growth factor (EGF) receptor and its ligands, EGF and transforming growth factor-β, in human fallopian tubes. Endocrinology 131:947–957; 1992.
Malayer, J. R.; Hansen, P. J.; Buhi, W. C. Secretion of proteins by cultured bovine oviducts collected from estrus through early diestrus. J. Exp. Zool. 248:345–353; 1988.
McNutt, T.; Rogowski, L.; Vasilatos-Younken, R., et al. Adsorption of oviductal fluid proteins by the bovine sperm membrane during in vitro capacitation. Mol. Reprod. Dev. 33:313–33; 1992.
Metzger, D. A.; Korach, K. S. Cell-free interaction of the estrogen receptor with mouse uterine nuclear matrix: Evidence of saturability, specificity, and resistance to KCl. Endocrinology 126:2190–2195; 1990.
Murphy, L. J.; Ghahary, A. Uterine insulin-like growth factor-1: Regulation of expression and its role in estrogen-induced uterine proliferation. Endocr. Rev. 11:443–453; 1990.
Murray, M. K. Biosynthesis and immunocytochemical localization of an estrogen-dependent glycoprotein and associated morphological alterations in the sheep ampulla oviduct. Biol. Reprod. 47:889–890; 1992.
Neulen, J.; Wagner, B.; Runge, M., et al. Effect of progestins, estrogens and antiestrogens on [3H]thymidine uptake by human endometrial and endosalpinx cells in vitro. Arch. Gynecol. 240:225–232; 1987.
Pollard, J. W.; Plante, C.; King, W. A., et al. Fertilizing capacity of bovine sperm may be maintained by binding to oviductal epithelial cells. Biol. Reprod. 44:102–107; 1991.
SAS. SAS/STAT User’s Guide, 4th ed., version 6. Cary, NC: SAS Inst.; 1990.
Savio, J. D.; Thatcher, W. W.; Badinga, L., et al. Regulation of dominant follicle turnover during the oestrous cycle in cows. J. Reprod. Fertil. 97:197–203; 1993.
Simmen, F. A.; Simmen, R. C. M.; Geisert, R. D., et al. Differential expression, during the estrous cycle and pre- and postimplantation conceptus development, of messenger ribonucleic acids encoding components of the pig uterine insulin-like growth factor system. Endocrinology 130:1547–1556; 1992.
Slayden, O. D.; Hirst, J. J.; Brenner, R. M. Estrogen action in the reproductive tract of rhesus monkeys during antiprogestin treatment. Endocrinology 132:1845–1856; 1993.
Takeuchi, K.; Nagata, Y.; Sandow, B. A., et al. Primary culture of human fallopian tube epithelial cells and co-culture of early mouse pre-embryos. Mol. Reprod. Dev. 32:236–242; 1992.
Walent, J. H.; Gorski, J. Estrogen binding is a noncooperative process in primary rat uterine cells. Endocrinology 126:383–391; 1990.
Watson, A. J.; Hogan, A.; Hahnel, A., et al. Expression of growth factor ligand and receptor genes in the preimplantation bovine embryo. Mol. Reprod. Dev. 31:87–95; 1992.
Weems, C. W.; Lee, C. N.; Weems, Y. S., et al. Distribution of progesterone to the uterus and associated vasculature of cattle. Endocrinol. Jpn. 35:625–630; 1988.
Wegner, C. C.; Killian, G. J. In vitro and in vivo association of an oviduct estrus-associated protein with bovine zona pellucida. Mol. Reprod. Dev. 29:77–84; 1991.
Author information
Authors and Affiliations
Additional information
Work conducted while on a sabbatical leave supported by the Deutsche Forschungsgemeinschaft.
Rights and permissions
About this article
Cite this article
Tiemann, U., Hansen, P.J. Steroidal and growth factor regulation of [3H]thymidine incorporation by cultured endosalpingeal cells of the bovine oviduct. In Vitro Cell Dev Biol - Animal 31, 640–645 (1995). https://doi.org/10.1007/BF02634318
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02634318