Summary
A rapid test has been developed based on the technique of latex immunoassay for the detection of Newcastle disease virus from suspected tissue suspensions. The latex particles were sensitised with globulins and were used for antigen detection. Of the 258 samples tested, 165 samples were positive by this kit which was compared for its efficacy with the standard OIE approved haemagglutination (HA) and haemagglutination inhibition (HI) tests. No significant difference (P > 0·05) was observed between the tests. The sensitivity and specificity of the developed test was 94·19% and 87·63% respectively.
Résumé
Un test rapide a été développé grâce à une technique immunologique au latex pour la détection du virus de la maladie de Newcastle chez des suspensions de tissus pouvant être infectés. Les particules de latex, sensibilisées avec des globulines furent utilisées pour la détection de l’antigène. Sur les 258 échantillons testés, 165 furent positifs avec ce type de kit dont l’efficacité fut comparée avec les méthodes standards de l’OIE: l’hémaglutination et le test d’inhibition de l’hémaglutination. Aucune différence ne fut significative (P > 0,05) entre les différents tests. La sensibilité et la spécificité furent respectivement pour ce test de 94,19% et 87,63%.
Resumen
Se desarrolló un test rápido basado en la técnica del immunoensayo en látex para la detección del virus de la enfermedad de Newcastle en suspensiones tisulares. Las partíiculas de latex se sensibilizaron con globulinas y se utilizaron para la detección de antígenos. De un total de 258 muestras analizadas, 165 fueron positivas de acuerdo con este test. La eficacia del test se comparó con los tests estándar de hemoaglutinación (HA) e inhibición de la hemoaglutinación (HI). No se encontraron diferencias entre tests. La sensibilidad y especificidad del test desarrollado fue del 94·19% y 87·63% respectivamente.
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Thirumurugan, G., Jayakumar, R., Kumanan, K. et al. Latex immunoassay for rapid detection of newcastle disease virus. Trop Anim Health Prod 29, 227–230 (1997). https://doi.org/10.1007/BF02632309
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DOI: https://doi.org/10.1007/BF02632309