Summary
A tandemly repeated DNA sequence present in several species of the family Brassicaceae has been characterized. Digestion of the genomic DNA from 17 species from this family revealed the presence of tandem repeats of about 175 bp monomeric size in 6 of the 11 investigated genera. All investigated genera within the tribe Brassiceae contained the sequence. TheBrassica napus Hind III 175-bp monomeric repeat unit was isolated and used as a probe in filter hybridizations to DNA from the other species. The sequence was found under highly stringent hybridization conditions in all of the sevenBrassica species analyzed, in the closely related genusEruca, and in the more distant genusCapsella. The generaRaphanus, Sinapis, andCrambe hybridized with the probe under moderately stringent conditions. The degree of hybridization increased with a further drop in hybridization stringency inRaphanus, Sinapis, andCrambe, indicating that sequence divergence has occurred in these genera relative toB. napus. The distribution of the various sequence multimers indicated a random and similar loss of restriction sites in all species hybridizing under high stringency. The copy number per haploid genome in theBrassica species varied between 1.7×105 and 3.0×105, except inB. nigra, where only 3.0×104 copies were found.
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Halldén, C., Bryngelsson, T., Säll, T. et al. Distribution and evolution of a tandemly repeated DNA sequence in the family brassicaceae. J Mol Evol 25, 318–323 (1987). https://doi.org/10.1007/BF02603116
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DOI: https://doi.org/10.1007/BF02603116