Abstract
Rationale and objectives: In experimental allergic encephalomyelitis (EAE), central nervous system (CNS) macrophage imaging is achievable by MRI using AMI-227 an ultra-small particle iron oxide contrast agent at a dose of 300 μmol/kg Fe. The objective was to test the feasibility at the human recommended dose of 45 μmol/kg Fe.Methods: Two groups of EAE rats were tested with AMI-227 using 45 and 300 μmol/kg Fe respectively. Following i.v. injection of AMI-227, they were scanned after a delay of 4–6 and 20–24 h.Results: With a high dose of AMI-227, all animals showed low signal intensity related to iron-loaded macrophages in the CNS. At low dose no abnormalities were found in the CNS. Furthermore, a delay of 4–6 h failed to demonstrate abnormalities even at high dose.Conclusions: Dose, scanning delay after administration and blood half-life are major parameters for T2* CNS macrophage imaging.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Weissleder R, Elizondo G, Wittenberg J, Lee AS, Josephson L, Brady TJ. Ultrasmall superparamagnetic iron oxide: characterization of a new class contrast agents for MR imaging. Radiology 1990;175:494–8.
Weissleder R, Cheng H-C, Bogdanova A, Bogdanov A Jr. Magnetically labeled cells can be detected by MR imaging. J Magn Reson Imaging 1997;7:258–63.
Anzai Y, Blackwell KE, Hirschowitz SL, Rogers JW, Sato Y, Yuh WTC, Runge VM, Morris MR, McLachan SJ, Lufkin RB. Initial clinical experience with dextran-coated superparamagnetic iron oxide for detection of lymph node metastases in patients with head and neck cancer. Radiology 1994;192:709–15.
Dousset V, Delalande C, Ballarino L, et al. In vivo macrophage activity imaging in the central nervous system detected by magnetic resonance. Magn Reson Med 1999;41:329–333.
Coyle PK. The neuroimmunology of multiple sclerosis. Adv Neuroimmunol 1996;6:143–54.
Batholdi D, Suchwab ME. Methylprednisolone inhibits early inflammatory processes but not ischemic cell death after experimental cord lesion in rat. Brain Res 1995;672:177–86.
Dubois-Dalcq M, Altmeyer R, Chiron M, Wilt S HIV interactions with cells of the nervous system. Curr Opin Neurobiol 1995;5:647–55.
Martin R, McFarland H. Immunological aspects of experimental allergic encephalomyelitis and multiple sclerosis. Crit Rev Clin Lab Sci 1995;32:121–82.
Rossi ML, Jones NR, Candy E, et al. The mononuclear cell infiltrate compared with survival in high grade astrocytomas. Acta Neuropathol (Berlin) 1989;78:189–93.
Polman CH, Dijkstra CD, Sminia T, Koetsier JC. Immunohistological analysis of the central nervous system of Lewis rats with experimental allergic encephalomyelitis. J Neuroimmunol 1986;11:215–22.
Bauer J, Huitinga I, Zhao W, Lassmann H, Hickey WF, Dijkstra CD. The role of macrophages, perivascular cells, and microglia cells in the pathogenesis of experimental autoimmune encephalomyelitis. Glia 1995;15:437–46.
Raine CS. Multiple sclerosis: immune system molecule expression in the central nervous system. J Neuropathol Exp Neurol 1994;53:328–37.
Chambon C, Clément O, Le Blanche A, Schouman-Claeys E, Frija G. Superparamagnetic iron oxides as positive MR contrast agents in vitro and in vivo. Magn Reson Imaging 1993;11:509–19.
Guimareas R, Clément O, Bittoun J, Carnot F, Frija G. MR lymphography with superparamagnetic iron nanoparticles in rats: pathologic basis for contrast enhancement. AJR 1994;162:201–7.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Dousset, V., Gomez, C., Petry, K.G. et al. Dose and scanning delay using USPIO for central nervous system macrophage imaging. MAGMA 8, 185–189 (1999). https://doi.org/10.1007/BF02594597
Issue Date:
DOI: https://doi.org/10.1007/BF02594597