Abstract
The aim of the study was to determine whether the induction of HSP70 by Zn2+ is able to protect the small bowel of rats against ischemia. Twenty-four male Wistar rats (weight 200–300 g) were divided into four groups: (1) saline treatment for 24 h (n=4); (2) Zn2+ treatment for 24 h (n=4); (3) Saline pretreatment for 24 h and ischemia (n=8); (4) Zn2+ pretreatment for 24h and ischemia (n=8). Pretreatment with Zn2+ was carried out by intraperitoneal administration of 50 mg/kg zinc bis (dl-hydrogen aspartate)=10 mg/kg Zn2+. Ischemia in a defined segment of the small bowel was produced by ligation of the mesenteric vein and artery and ligation of both ends of the segment. Tissue samples were collected before and 2, 4 and 6 h after ligation and investigated by histology, immunohistochemistry and Western blotting. Twenty-four h after i.p. Zn2+ injection, the small bowel expressed increased HSP70 tissue levels. Histology with subsequent grading of ischemic tissue injury showed significantly decreased tissue necrosis after Zn2+ pretreatment and HSP70 induction compared with saline pretreated controls. In conclusion, this study proves that Zn2+ is inducing HSP70 in the small bowel in vivo and hereby able to protect the small bowel against ischemia.
Zusammenfassung
Ziel dieser Studie war es zu klären, ob die HSP70-Induktion durch Zn2+ den Dünndarm von Ratten gegen Ischämie schützen kann. Es wurden 24 männliche Wistar-Ratten (Gewicht 200–300 g) in 4 Gruppen aufgeteilt: I. NaCl-Behandlung über 24 h (n=4); II. Zn2+-Behandlung über 24 h (n=4); III. NaCl-Vorbehandlung über 24 h und Ischämie (n=8); IV. Zn2+-Vorbehandlung über 24 h und Ischämie (n=8). Die Zn2+-Vorbehandlung erfolgte durch intraperitoneale Gabe von 50 mg/kg Zink-di-dl-Hydrogenaspartat=10 mg/kg Zn2+. Die Ischämie in einem definierten Segment des Dünndarms wurde durch eine Ligatur der V. und A. mesenterica sowie der beiden Segmentenden erzeugt. Gewebeproben wurden jeweils vor und 2, 4 und 6 h nach der Ligatur histologisch, immunohistochemisch und durch «Western blotting» untersucht. 24 h nach intraperitonealer Zn2+-Injektion zeigten sich erhöhte HSP70-Dünndarmgewebespiegel. Histologische Untersuchungen mit nachfolgender Klassifizierung des Ischämieschadens ergaben geringere Gewebenekrotisierung nach Zn2+-Vorbehandlung sowie HSP70-Induktion im Vergleich zu den mit NaCl vorbehandelten Kontrolltieren.Schlußfolgerung: Diese Studie weist nach, daß Zn2+ im Dünndarm in vivo (bei Ratten) HSP70 induzieren kann und daß damit der Dünndarm gegen Ischämie geschützt werden kann.
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Töns, C., Klosterhalfen, B., Klein, H.M. et al. Induction of heat shock protein 70 (HSP70) by zinc bis (dl-hydrogen aspartate) reduces ischemic small-bowel tissue damage in rats. Langenbecks Arch Chiv 382, 43–48 (1997). https://doi.org/10.1007/BF02539308
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DOI: https://doi.org/10.1007/BF02539308