Abstract
Phosphatidylethanolamine (PE) and phosphatidylglycerol (PG) were isolated from aVibrio species of bacterium, known to produce eicosapentaenoic acid (20∶5n−3) andtrans-hexadecenoic acid (16∶1n−7), and subjected to phospholipase A2 degradation to determine the positional distribution of component fatty acids. At the two growth temperatures studied (20 and 5°C), both 20∶5n−3 andtrans 16∶1 n−7 were located mainly at positionsn−2 in PE. Increases in the proportions of 20∶5n−3 andtrans 16∶1n−7 in positionsn−2 with decreasing growth temperature were balanced mainly by decreases in the level ofiso-15∶0. In PG,trans 16∶1n−7 was located predominantly in positionsn-1, although the difference between the two positions was not as great as in PE. Eicosapentaenoic acid was preferentially located in positionsn-2 of PG, particularly at 5°C when it comprised 29.9% of the total fatty acids in this position. It is concluded thattrans 16∶1n−7/20∶5n−3 is not a major molecular species of phospholipid in this species ofVibrio and that changes in the levels of molecular species of PE containingiso-15∶0 may feature in thermal acclimation.
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Abbreviations
- HPLC:
-
high-performance liquid chromatography
- HPTLC:
-
high-performance thin-layer chromatography
- PE:
-
phosphatidylethanolamine
- PG:
-
phosphatidylglycerol
- PUFA:
-
polyunsaturated fatty acids
- TLC:
-
thin-layer chromatography
- UV:
-
ultraviolet
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Henderson, R.J., Millar, R.M. & Sargent, J.R. Effect of growth temperature on the positional distribution of eicosapentaenoic acid andrans hexadecenoic acid in the phospholipids of aVibrio species of bacterium. Lipids 30, 181–185 (1995). https://doi.org/10.1007/BF02538274
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DOI: https://doi.org/10.1007/BF02538274