Abstract
TheERG6 gene fromSaccharomyces cerevisiae has been functionally expressed inEscherichia coli, for the first time, yielding a protein that catalyzes the bisubstrate transfer reaction whereby the reactive methyl group from (S)-adenosyl-l-methionine is transferred stereoselectively to C-24 of the sterol side chain. The structural requirements of sterol in binding and catalysis were similar to the native protein fromS. cerevisiae. Inhibition of biomethylation was observed with fecosterol and ergosterol which suggests that ergosterol may function in wild-type yeast as a feedback regulator of sterol biosynthesis.
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Abbreviations
- AdoMet:
-
(S)-adenosyl-l-methionine
- HPLC:
-
high-performance liquid chromatography
- ORF:
-
open reading frame
- SMT:
-
(S)-adenosyl-l-methionine:Δ24(25)
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Venkatramesh, M., Guo, Da., Harman, J.G. et al. Sterol specificity of theSaccharomyces cerevisiae ERG6 gene product expressed inEscherichia coli . Lipids 31, 373–377 (1996). https://doi.org/10.1007/BF02522922
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DOI: https://doi.org/10.1007/BF02522922