Summary
The possibility of determination of four cephalosporin antibiotics in clinical samples by capillary electrophoresis has been investigated. The separation conditions for capillary zone electrophoresis (CZE) were studied in detail. The precision of migration times measured by use of the optimized method was satisfactory (RSD<1%) and response was linearly dependent on concentration over the approximate range 2–150 mg L−1 for all the compounds studied (cefuroxime, cefotaxime, ceftriaxone, and ceftazidime). Complete separation could be achieved within 5 min. The CZE method was found to be highly suitable for direct determination of the antibiotics in clinical samples such as wound drainage, cerebrospinal fluid, and urine; for serum, however, the use of micellar electrokinetic capillary chromatography (MECC) was more advantageous.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Altria, K.D.Analysis of Pharmaceuticals by Capillary Electrophoresis, Vieweg, Braunschweig,1998.
Honda, S.; Taga, A.; Kakehi, K.; Koda, S.; Okamoto, Y.J. Chromatogr. 1992,590, 364–368.
Fabre, H.; Penalvo, P.G.J. Liq. Chromatogr. 1995,18, 3877–3887.
Mrestani, Y.; Neubert, R.; Schiewe, J.; Hartl, A.J. Chromatogr. B 1997,690, 321–326.
Nishi, H.; Tsumagari, N.; Kakimoto, T.; Terabe, S.J. Chromatogr. 1989,477, 259–270.
Emaldi, P.; Fapanni, S.; Baldini, A.J. Chromatogr. A 1995,711, 339–346.
Penalvo, P.G.; Julien, E.; Fabre, H.Chromatographia 1996,42, 159–164.
Pajchel, G.; Tyski, S.J. Chromatogr. A 2000,895, 27–31.
Penalvo, P.; Kelly, M.; Maillols, H.; Fabre, H.Anal. Chem. 1997,69, 1364–1369
Schmutz, A.; Thormann, W.Electrophoresis,1994,15, 1295–1303.
Lloyd, D.K.; Watzig, H.J. Chromatogr. B 1995,663, 400–405.
Gáspár, A.; Andrási, M.; Kardos, S.Chromatographia submitted for publication.
Honda, S.; Taga, A.; Kakehi, K.; Koda, S.; Okamoto, Y.J. Chromatogr. 1992,590, 364–368.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gáspár, A., Kardos, S., Andrási, M. et al. Capillary electrophoresis for the direct determination of cephalosporins in clinical samples. Chromatographia 56, S109–S114 (2002). https://doi.org/10.1007/BF02494122
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02494122