Summary
A fast and rabust method for the determination of 1-hydroxypyrene (OH-pyrene) and other hydroxylated PAHs in urine by stir bar sorptive extraction (SBSE) combined with thermal desorption-capillarygas chromatography-mass spectrometry (TD-CGC-MS) is described. After enzymatic hydrolysis, in-situ derivatisation with acelic acid anhydride precedes SBSE sampling to improve the chromatographic behavior of the analytes. The performance of the method has been evalucted by the quantitative determination of OH-pyrene in some certified samples. Detection limits as low as 2ngL1(ppt) can be obtained. The procedure is illustrated with the analysis of a urine sample of a firefighter.
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Desmet, K., Tienpont, B. & Sandra, P. Analysis of 1-hydroxypyrene in urine as PAH exposure marker using in-situ derivatisation stir bar sorptive extraction-thermal desorption-capillary gas chromatography-mass spectrometry. Chromatographia 57, 681–685 (2003). https://doi.org/10.1007/BF02491749
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DOI: https://doi.org/10.1007/BF02491749