Summary
A commercial reversed-phase (RP) C18 HPLC column has been dynamically coated with the chiral selectorN τ-n-decyl-l-spinacine and then loaded with copper(II) ions. Several racemic mixtures of underivatized amino acids and oligopeptides were resolved on the column by chiral ligand-exchange chromatography. The most important experimental conditions affecting column efficiency, retention, and selectivity (temperature and mobile phase flow rate and composition) were extensively investigated.
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References
Allenmark, S.G.,Chromatographic Enantioseparation: Methods and Applications, Ellis Horwood, Chichester,1988.
Krstulovic, A.M. Ed.Chiral Separations by HPLC: Applications to Pharmaceutical Compounds Ellis Horwood, Chichester,1989.
Ahuia, S. Ed.,Chiral Separations by Liquid Chromatography ACS Symposium Series 471, American Chemical Society, Washington, DC,1991.
Subramanian, G. Ed.,A Practical Approach to Chiral Separations by Liquid Chromatography, VCH, Weinheim,1994.
Beesley, T.E.; Scott, R.P.W.Chiral Chromatography John Wiley and Sons, Chichester,1998.
Davankov, V.A.; Navratil, J.D.; Walton, H.F.Ligand Exchange Chromatography CRC Press, Boca Raton, Florida,1988.
Rogozhin, S.V.; Davankov, V.A.Chem. Commun. 1971, 490.
Lepage, J.; Lindner, W.; Davies, G.; Seitz, D.; Karger, B.L.Anal. Chem. 1979,51, 433–435.
Lindner, W.; Lepage, J.; Davies, G.; Seitz, D.; Karger, B.L.J. Chromatogr. 1979,185, 323–344.
Tapuhi, Y.; Miller, N.; Karger, B.L.J. Chromatogr. 1981,205, 325–337.
Remelli, M.; Fornasari, P.; Pulidori, F.J. Chromatogr. A 1997,761, 79–89, and references cited therein.
Wachsmann, M.; Bruckner, H.Chromatographia 1998,47, 637–642.
Wan, Q.H.; Shaw, P.N.; Davies, M.C.; Barrett, D.A.J. Chromatogr. 1997,786, 249–257.
Miyazawa, T.; Minowa, H.; Imagawa, K.; Yamada, T.Anal. Lett. 1997,30, 867–882.
Yamazaki, S.; Saito, K.; Tanimura, T.J. High Resol. Chromatogr. 1998,21, 561–564.
Chilmonczyk, Z.; Ksycinska, H.; Cybulski, J.; Rydzewski, M.; Les, A.Chirality 1998,10, 821–830.
Chen, Z.L.; Lin, J.M.; Uchiyama, K.; Hobo, T.Anal. Sci. 2000,16, 131–137.
Remelli, M.; Piazza, R.; Pulidori, F.Chromatographia 1991,32, 278–284.
Remelli, M.; Fornasari, P.; Dondi, F.; Pulidori, F.Chromatographia 1993,37, 23–30.
Borghesani, G.; Pulidori, F.; Remelli, M.; Purrello, R.; Rizzarelli, E.J. Chem. Soc., Dalton Trans. 1990, 2095–2100.
Remelli, M.; Rossi, S.; Guerrini, R.; Pulidori, F.Ann. Chim. (Rome) 1995,85, 503–518.
Remelli, M.; Pulidori, F.; Guerrini, R.; Bertolasi, V.J. Chem. Crystallogr. 1997,27, 507–513.
Remelli, M.; Faccini, S.; Conato, C.J. Chromatogr. submitted.
Rizzi, A.M.J. Chromatogr. 1991,542, 221–237.
Davankov, V.A.J. Chromatogr. A 1994,666, 55–76.
Arena, G.; Conato, C.; Contino, A.; Pulidori, F.; Purrello, R.; Remelli, M.; Tabbi, G.Ann. Chim. (Rome) 1998,88, 1–12.
Remelli, M.; Conato, C.; Agarossi, A.; Pulidori, F.; Mlynarz, P.; Kozlowski, H.Polyhedron 2000,19, 2409–2419.
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Remelli, M., Trombin, D. & Conato, C. Chiral ligand-exchange chromatography on an RP HPLC column coated with a new chiral selector derived froml-spinacine. Chromatographia 55, 301–306 (2002). https://doi.org/10.1007/BF02491663
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DOI: https://doi.org/10.1007/BF02491663