Summary
The separation of mono- and divinyl chlorophyllc forms was estudied employing several octylsilica and octadecylsilica columns under isocratic conditions. In this paper we show that, using the adequate mobile phases, the monomeric C8 columns can separate the mentioned chlorophylls. A comparison between C8 and C18 columns reveals that monomeric OS phases provide always higher resolution of the MV- and DV chlorophyllc pairs. Such a result could be explained in terms of differences in column polarity. When adequate gradient profiles and injection conditions are used the separation is accomplished together with that of other chlorophylls and carotenoids.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
H. Scheer, in H. Scheer (Editor), Chlorophylls, CRC Press, Boca Raton, 1991, p. 3.
K. Saitoh, H. Kato, N. Teramae, J. Chromatogr. A687, 149 (1994).
H. Kato, K. Saitoh, N. Teramae, Chem. Lett. (7), 547, (1995).
Y. Shioi, S. I. Beale, Anal. Biochem.162, 493 (1987).
S. W. Jeffrey, in J. C. Green, B. S. C. Leadbeater and W. L. Diver, (Editors), The Chromophyte Algae: Problems and Perspectives, Clarendon Press, Oxford, 1989, p. 13.
K. Saitoh, I. Awaka, N. Suzuki, J. Chromatogr. A653, 247 (1993).
K. Saitoh, I. Awaka, N. Suzuki, J. Chromatogr. A693, 176 (1995).
R. F. C. Mantoura, C. A. Llewellyn, Anal. Chim. Acta151, 297 (1983).
M. Zapata, A. M. Ayala, J. M. Franco, J. L. Garrido, Chromatographia23, 26 (1987).
S. W. Wright, S. W. Jeffrey, R. F. C. Mantoura, C. A. Llewellyn, T. Bjørnland, D. Repeta, N. Welschmeyer, Mar. Ecol. Prog. Ser.77, 183 (1991).
G. W. Kraay, M. Zapata, M. J. Veldhuis, J. Phycol.28, 708 (1992).
L. Van Heukelem, A. J. Lewitus, T. M. Kana, N. E. Craft, J. Phycol.28, 867 (1992).
J. L. Garrido, M. Zapata, Chromatographia35, 543 (1993).
J. L. Garrido, M. Zapata, J. High Resolut. Chromatogr.16, 229 (1993).
L. Van Heukelem, A. J. Lewitus, T. M. Kana, N. E. Craft, Mar. Ecol. Prog. Ser.114, 303 (1994).
K. Van Lenning, J. L. Garrido, J. Aristegui, M. Zapata, Chromatographia41, 539 (1995).
J. L. Garrido, M. Zapata, Chromatographia44, 43 (1997).
R. Goericke, D. Repeta, Mar. Ecol. Prog. Ser.101, 307 (1993).
G. B. McManus, R. Dawson, Mar. Ecol. Prog. Ser.113, 199 (1994).
R. G. Barlow, R. F. C. Mantoura, D. G. Cummings, T. W. Fileman, Deep-Sea Res. II,44, 833 (1997).
F. Vidussi, H. Claustre, J. Bustillos-Guzmàn, C. Cailliau, J-C. Marty, J. Plankton Res.18, 2377 (1996).
R. G. Barlow, D. G. Cummings, S. W. Gibb, Mar. Ecol. Prog. Ser.161, 303 (1997).
L. C. Sander, S. A. Wise, LC.GC.,8, 378 (1990).
L. C. Sander, S. A. Wise, Anal. Chem.56, 504 (1984).
J. L. Garrido, M. Zapata, S. Muñiz, J. Phycol.31, 761 (1995).
J. L. Garrido, M. Zapata, J. Phycol.34, 70 (1998).
M. Zapata, J. L. Garrido, Chromatographia31, 589 (1991).
P. E. Antle, L. R. Snyder, LC Mag.2, 840 (1984).
L. R. Snyder, J. L. Glajch, J. J. Kirkland, Practical HPLC Method Development, John Wiley & Sons, New York, 1988.
M. Zapata, J. L. Garrido, J. Phycol.33, 209 (1997).
C. A. Rebeiz, R. Parham, D. A. Fasoula, I. M. Ioannides in D. J. Chadwick and K. Ackrill (Editors), The Biosynthesis of the Tetrapyrrole Pigments, John Wiley & Sons, Chichester, 1994, p. 177.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rodríguez, F., Zapata, M. & Garrido, J.L. High performance liquid chromatographic separation of chlorophyllc forms from marine phytoplankton on octylsilica bonded phases. Chromatographia 48, 677–680 (1998). https://doi.org/10.1007/BF02467598
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02467598