Abstract
In vitro culture ofBrassica alba anthers on a growth medium containing inorganics of KB5 and organics, iron, sucrose and hormones of B5 resulted in a very high response of anthers (93.75%) towards callus induction. All the calli transferred to regeneration media responded favourably even after six months of callus induction. Numerous torpedo-shaped embryoids developed in clusters at many sites from each callus mass. Secondary embryogenesis and multiple shoot formation was also observed in many cases. The number of embryoids and plantlets produced by one embryogenic anther were as high as 169.8 and 17 respectively. 87% of the regenerated plants were haploids.
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Abbreviations
- SH:
-
Schenk and Hildebrandt, 1972
- B5:
-
Gamborg et al. 1968
- NN:
-
Nitsch and Nitsch, 1969
- N6:
-
Chu et al. 1975
- MS:
-
Murashige and Skoog, 1962
- BL:
-
Blaydes, 1966
- DBM II:
-
Gresshoff and Doy, 1972
- KB5:
-
Keller et al. 1975
- BAP:
-
6-benzyladenine
- 2, 4-D:
-
2, 4-dichlorophenoxyacetic acid
- p-CPA-p:
-
chlorophenoxyacetic acid
- Kin:
-
Kinetin
- NAA:
-
naphthaleneacetic acid
- IAA:
-
indoleacetic acid
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Communicated by I.K. Vasil
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Leelavathi, S., Reddy, V.S. & Sen, S.K. Somatic cell genetic studies inBrassica species. Plant Cell Reports 3, 102–105 (1984). https://doi.org/10.1007/BF02441010
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DOI: https://doi.org/10.1007/BF02441010