Abstract
Solving the experimental difficulties associated with measurement of the electrical impedance of living tissues gives access to valuable tissue compartment parameters which are sensed within seconds using minimally invasive, simple metallic electrodes. Extracellular conductivity and cell membrane capacitance can be followed over time under conditions of metabolic toxicity, perfusion loss and thermal stress in liver, brain cortex, and muscle, respectively. Application of this technique in burns therapy allows an accurate estimation of the severity of thermal injury to skeletal muscle, supporting predictions on tissue survival.
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Armstrong, B. Preliminary analysis of C/R and temperature data from rats. Canadian Electrical Association research report 165-D-286A: Appendix X; 1992.
Bao, J.-Z.; Davis, C.C.; Schmukler, R.E. Frequency domain impedance measurements of erythrocytes. Biophys. J. 61:1427–1434; 1992.
Cherkasov, L.V.; Davletchina, R.F. Ultrastructure of the cerebral cortical neurons in hypoxic hypoxia. Zh. Nevropatol. Psikhiatr. 88(7):16–19; 1988.
Cohadon, F. Physiopathologie des oedèmes cérébraux. Rev. Neurol. (Paris) 143(1):3–20; 1987.
Doll, C.J.; Hochachka, P.W.; Reiner, P.B. Effects of anoxia and metabolic arrest on turtle and rat cortical neurons. Am. J. Physiol. 260:R747-R755; 1991.
Espinoza, M.I.; Parer, J.T. Mechanisms of asphyxial brain damage, and possible pharmacologic interventions, in the fetus. Am. J. Obstet. Gynecol. 164:1582–1589; 1991.
Evans, D.; Héroux, P. EIS as a diagnostic aid in the evaluation of high-voltage electrical injury. Canadian Electrical Association research report 165-D-286A: Appendix XI; 1992.
Fishman, H.M.; More, L.E.; Poussart, D. The biophysical approach to excitable membranes. New York: Plenum Publishing, pp. 65–95; 1981.
Fricke, H. The theory of electrolytic polarization. Phil. Mag. 14:310–318; 1932.
Gabbiani, G.; Badonnel, M.C. Early changes of endothelial clefts after thermal injury. Microvascular Research 10:65–75; 1975.
Hahn, G. Metabolic aspects of the role of hyperthermia in mammalian cell inactivation and their possible relevance to cancer treatment. Cancer Res. 34:3117–3123; 1974.
Halsey, T.C.; Leibig, M. Random walks and the doublelayer impedance. Europhys. Lett. 14(8):815–820; 1991.
Hansen, A.J. Ion and membrane changes in the brain during anoxia. Behav. Brain Res. 14(2):93–98; 1984.
Henle, K.J. Arrhenius analysis of thermal responses. In: Hyperthermia in Cancer Therapy. G.K. Hall Medical Publishers; 1983: pp. 47–53.
Héroux, P. Thermal damage: mechanisms, patterns and detection in electrical burns. In: Lee, Cravalho, Burke, eds. Electrical Trauma. Cambridge University Press, in press
Héroux, P.; Ballard, P.A.; Daniel, R.K.; Howard, C.R. Studies on electrical burns. San Francisco: Seventh annual meeting of the Bioelectromagnetics Society; June 1985; 5 p.
Héroux, P.; Ballard, P.A.; Daniel, R.K.; Howard, C.R.; Zelt, R.G. Experimental investigation of electrical burns: analysis of recorded temperatures. In: Rudolf Hauf, ed. Beiträge zur Ersten Hilfe und Behandlung von Unfällen durch elektrischen Strom. Freiburg. W. Germany; 1986; pp. 147–180.
Homblé, F.; Ferrier, J.M. Analysis of the diffusion theory of negative capacitance: the role of K+ and the unstirred layer thickness. J. Theor. Biol. 131(2):183–197; 1988.
Iaizzo, P.A.; Olsen, R.A.; Seewald, M.J.; Powis, G.; Stier, A.; Van Dyke, R.A. Transient increases of intracellular Ca2+ induced by volatile anesthetics in rat hepatocytes. Cell Calcium 11(8):515–524; 1990.
Lazarevich, E.V.; Samoilov, M.O.; Semenov, D.G. Changes in the calcium metabolism of cerebral cortical structures in anoxia in vitro. Biull. Eksp. Biol. Med. 105(3):261–264; 1988.
Liu, S.H.; Kaplan, T.; Gray, L.J. Ac response of fractal interfaces. Solid State Ionics 18 & 19:65–71; 1986.
Macpherson, A.I.S.eaet al. The spleen. Springfield, Ill.: Charles C. Thomas; 1973.
McLean, A.E.M.; McLean, E.; Judah, J.D. Cellular necrosis in the liver induced and modified by drugs. Int. Rev. Exp. Pathol. 4:127–157; 1965.
McNeil, P.L. Cell wounding and healing. American Scientist 79:222–235; 1991.
Miyamoto, S.; Fishman, H.M. Linear analysis of K conduction in snail neuron from impedance determinations. Ferroelectrics 86:129–146; 1988.
Mubarak, S.; Owen, C.A. Compartmental syndrome and its relation to the crush syndrome: a spectrum of disease. Clinical Orthopaedics and Related Research 113:81–89; 1975.
Quinby, W.C.; Burke, J.F.; Trelstad, R.L.; Caulfield, J. The use of microscopy as a guide to primary excision of high-tension electrical burns. Journal of Trauma 18(6):423–431; 1978.
Rees K.R. Cellular injury by drugs. Mongar, J.L.; de Reuck, A.V.S., eds. Ciba Foundation Symposium on Enzymes and Drug Action. Boston: Little Brown & Co; 1962: pp. 344–358.
Reiner, P.B.; Laycock, A.G.; Doll, C.J. A pharmacological model of ischemia in the hippocampal slice. Neurosci. Lett. 119(2):175–178; 1990.
Sapoval, B.; Chazalviel, J.-N. Electrical response of fractal and porous interfaces. Physical Review A 38(11):5867–5887; 1988.
Schwann, H.P. Alternating current electrode polarization. Biophysik 3:181–201; 1966.
Smith, G.S. The electric-field probe near a material interface with application to the probing of fields in biological bodies. IEEE Trans. On Microwave Theory and Tech., vol. MTT-27, no. 3, 270–278, 1979.
Wang, J.C. Construction of cpa admittance with distributions of series rc-pairs. Solid State Ionics 39:277–281; 1991.
Weinachter, S.N.; Blavet, N.; O'Donnell, R.A.; MacKenzie, E.T.; Rapin, J.R. Models of hypoxia and cerebral ischemia. Pharmacopsychiatry 23 Suppl. 2:94–98; 1990.
Zelt, R.G.; Ballard, P.A.; Héroux, P.; Daniel, R.K. Experimental high voltage electrical burns: the role of progressive necrosis. Plast. Surg. Forum 9:220–222; 1986.
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Héroux, P., Bourdages, M. Monitoring living tissues by electrical impedance spectroscopy. Ann Biomed Eng 22, 328–337 (1994). https://doi.org/10.1007/BF02368239
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DOI: https://doi.org/10.1007/BF02368239