Abstract
Twenty adult male rats per group in 4 treatment groups were injected intraperitoneally at 08.00 hours with 0.1 ml of an aqueous cotton seed extract (Gossypium barbadense Linn.) (Malvaceae) in concentrations of (a) 105.25, (b) 21.21, (c) 4.65, (d) 2.325 mg ml-1 (kg body weight)-1, respectively. A fifth group (control) was given 0.1 ml of pyrogen free distilled water per rat. Five rats per treatment group were sacrificed at 2, 8, 24 and 168 hours respectively after treatment. Plasma follicle stimulating hormone (FSH) and luteinizing hormone (LH) showed no change. Plasma testosterone was lower (p<0.05) than that of control at 2 and 8 hours, with recovery by 168 hours post treatment. Plasma creatinine was raised by 2 hours, with recovery by 8 hours. Plasma urea rose gradually but persistently to a maximum of 168 hours. Plasma aspartate (AST) and alanine (ALT) transaminases were significantly higher (p<0.001) than that of controls throughout the study. Testicular histology showed early germ cell disorganization followed by progressive fibrosis (sperm cytoskeleton) by 24 hours. There was evidence of recovery by 168 hours. It is concluded that aqueous extract of cotton seed meal contains substances that can rapidly cause damage to testicular, liver, kidney and muscular tissues.
Resumé
On a injecté à 8 heures par voie intrapéritonéale à 20 rats mâles, divisés en 4 groupes de traitement, 0,1 ml d'une solution aqueuse d'extrait de graine de coton (Gossypium barbedense Linn) (Malvacée) en concentrations respectives de (a) 105,25, (b) 21,21, (c) 4,65, et (d) 2,325 mg/ml/kg de poids corporel. On a administré, par rat, à un cinquième groupe (témoins) 0,1 ml d'eau distillée exempte de pyrogène. Dans chaque groupe de traitement, 5 rats ont été sacrifiés respectivement à 2, 8, 24 et 168 heures après le traitement. Aucun changement n'a été constaté en ce qui concernait l'hormone folliculostimulante (FSH) et l'hormone lutéinisante (LH) plasmatiques. La testostérone plasmatique était moins élevée (p<0,05) que chez les témoins à 2 et 8 heures, mais était remontée 168 heures après le traitement. La créatinine plasmatique s'était accrue 2 heures après, mais avait retrouvé son niveau d'origine après 8 heures. L'urée plasmatique augmentait progressivement mais de façon persistante jusqu'à une maximum à 168 heures. Les transaminases plasmatiques d'aspartate (AST) et d'alanine (ALT) étaient significativement plus élevées (p<0,001) que chez les témoins pendant toute la durée de l'étude. L'histologie testiculaire a révélé une désorganisation précoce des cellules germinales, suivie d'une fibrose progressive (cytosquelette du sperme) après 24 heures. On a constaté des signes de retour à la normale après 168 heures. Cette étude a permis de conclure qu'une solution aqueuse d'extrait de graine de coton contient des substances qui peuvent rapidement entraîner des dégats dans les tissus testiculaires, hépatiques, rénaux et musculaires.
Resumen
Veinte ratas macho adultas por grupo, en 4 grupos de tratamiento, fueron inyectadas por vía intraperitoneal a las 8 horas con 0,1 ml de una solución acuosa de extracto de semillas de algodón (Gossypium barbedense Linn) (malvácea) en concentraciones de (a) 105,25, (b) 21,21, (c) 4,65 y (d) 2,325 mg/ml/kg de peso corporal respectivamente. Se administró, por rata, a un quinto grupo (testigos) 0,1 ml de agua destilada libre de pirógeno. En cada grupo de tratamiento, 5 ratas fueron sacrificadas respectivamente a las 2, 8, 24 y 168 horas de la iniciación del tratamiento. No se verificó ningún cambio en lo que respecta a la hormona foliculoestimulante (FSH) y a la hormona luteinizante (LH) plasmáticas. La testosterona plasmática fue menos elevada (p<0,05) que en los testigos a las 2 y 8 horas, con recuperación a las 168 horas de iniciado el tratamiento. La creatinina plasmática se elevó a las 2 horas, pero recuperó su nivel original al cabo de 8 horas. La urea plasmática aumentó progresiva pero persistentemente a un nivel máximo a las 168 horas. Las transaminasas plasmáticas aspartate (AST) y alanina (ALT) fueron significativamente más altas (p<0,001) que en los testigos durante todo el curso del estudio. La histología testicular reveló una desorganización precoz de las células germinales, seguida de una fibrosis progresiva (cistoesqueleto del esperma) al cabo de 24 horas. Se observaron signos de retorno a lo normal al cabo de 168 horas. Se pudo llegar a la conclusión de que el extracto de semillas de algodón contiene sustancias que puedon dañar rápidamente los tejidos testiculares, hepáticos, renales y musculares.
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References
Mayes, P.A. (1988). Lipids of physiologic significance. In:Harpers Biochemistry, R.K.Murray, D.K.Granner, P.A.Mayes and V.W.Rodwell, eds, 21st edition. Appleton and Lange, California, pp. 130–141
National Coordinating Group on Male Antifertility Agents (1978). Gossypol: A new antifertility agent for males.Chin. Med. J.,4, 417–428
Conway, G.A. and Slocumb, J.C. (1979). Plants used as abortifacients and emmenagogues by Spanish New Mexicans.J. Ethnopharmacol.,1, 241
Casey, R.C.D. (1960). Alleged antifertility plants of India.Ind. J. Med. Sci.,14, 590–600
Prasad, M.R.N. and Diczfalusy, E. (1982). Physiology and medical aspects of andrology. ‘Gossypol’.Int. J. Androl. (Suppl.),5, 53–70
DePeyster, A., Quintanilha, A., Packer, L. and Smith, M.T. (1984). Oxygen radical formation induced by gossypol in rat liver microsomes and human sperm.Biochem. Biophys. Res. Commun.,118, 573–579
Edwards, J.G. (1986). Gossypol and rat germinal cells.Res. Reprod.,18, 4
Reyes, J., Aallen, J., Tanphaichitr, N., Bellve, A.R. and Benos, D.J. (1984). Molecular mechanisms of gossypol action on lipid membranes.J. Biol. Chem.,259, 9607–9615
Baccetti, B., Bigliardi, E., Burrini, A.G., Renieri, T. and Selmi, G. (1986). The action of gossypol on rat germinal cells.Gamete Res.,13, 1–7
Furayama, S., Mayes, D.M. and Nugent, C.A. (1970). A radioimmunoassay for plasma testosterone.Steroids,16, 415–428
Varley, H. (1967)Practical Clinical Chemistry. Interscience Publishers, Inc., New York, pp. 158–167
Giörgio, J.D. (1974). Manual determination of creatinine and creatine. In:Clinical Chemistry Principles and Techniques. R.J.Henry, D.C.Cannon and J.W.Winkelman, eds, 2nd edition. Harper and Row, New York, pp. 543–552
Henry, R.J., Chiamori, N., Golub, O.J. and Berkman, S. (1960). Revised spectrophotometric methods for the determination of glutamic-oxalacetic transaminase, glutamic-pyruvic transaminase and lactic acid dehydrogenase.Am. J. Clin. Pathol.,34, 381–398
Kirk, R.E. (1982).Experimental Design: Procedures for the Behavioral Sciences, 2nd Edition. Brooks/Cole Publishing Company, USA, p. 911
Xue, S.P., Zong, S.D., Su, S.Y., Wu, Y., Lih Zhou, Z.H. and Ma, X.X. (1980). Antifertility effect of gossypol on the germinal epithelium of the rat testis: a cytological autoradiographical and ultrastructural observation.Sci. Sin. (English edition),23, 642–657
Xue, S.P. (1981). Studies on the antifertility effect of gossypol, a new contraceptive for males. In:Recent Advances in Fertility Regulation. Proceedings of a Symposium. Beijin, China. C.F.Chang, D.Griffin and A.Wollman, eds, S.A. Atar, Geneva, pp. 122–146
Tang, X.C., Shu, M. and Shi, Q.X. (1980). Comparative studies on the absorption, distribution and excretion of14C-gossypol in four species of animals.Acta Pharmacol. Sing.,15, 212–217
Baron, D.N. (1985). The kidneys. In:A Short Textbook of Chemical Pathology, 4th edition. ELBS Edward Arnold, Great Britain, pp. 207–228
Hadley, M.A., Liu, C.Y. and Dym, M. (1981). Effects of gossypol on the reproductive system of male rats.Int. J. Androl.,2, 190–194
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Thomas, K.D., Caxton-Martins, A.E., Elujoba, A.A. et al. Effects of an aqueous extract of cotton seed (Gossypium barbadense Linn.) on adult male rats. Adv Contracept 7, 353–362 (1991). https://doi.org/10.1007/BF02340182
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DOI: https://doi.org/10.1007/BF02340182