Abstract
Two different satellite DNAs from tenebrionid speciesTribolium madens (Insecta, Coleoptera) have been detected, cloned, and sequenced. Satellite I comprises 30% of the genome; it has a monomer size of 225 by and a high A + T content of 74%. Satellite 11, with a monomer size of 711 by and A + T content of 70%, is less abundant, making 4% of the total DNA. Sequence variability of the monomers relative to consensus sequence is 4.1% and 1.2% for satellite I and II, respectively. Both satellites are localized in the heterochromatic regions of all chromosomes. A search for internal motifs showed that both satellites contain a related subsequences, about 100 by long. The creation of satellite I monomer is explained by duplication of the basic subunit, followed by subsequent divergence by single point mutations, deletions, and gene conversion. Inversion of the subsequence in addition to its duplication has occurred in satellite II. The result of this inversion is possible formation of a long, stable dyad structure. The 408-bp sequence, inserted within satellite II monomer, shares no similarity with a basic subunit. Frequent direct repeats found within the inserted sequence point to its evolution by duplication of shorter motifs. It is proposed that both satellites have been derived from a common ancestral sequence whose duplication played a major role in the formation of satellite I monomer, while insertion of a new sequence together with inversion of an ancestral one induced the occurrence of satellite II.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Bachmann L, Sperlich D (1993) Gradual evolution of a specific satellite DNA family inDrosophila ambigua, D. tristis andD. obscura. Mol Biol Evol 10:647–659
Benson D, Lipman DJ, Osrell J (1993) Genbank. Nucleic Acids Res 21:2963–2965
Bigot Y, Hamelin MH, Periquet G (1990) Heterochromatin condensation and evolution of unique satellite DNA families in two parasitic wasp species:Diadromus pulchellus andEupelmus vuilleti (Hymenoptera). Mol Biol Evol 7:351–364
Fowler RF, Skinner DM (1985) Cryptic satellites rich in inverted repeats comprise 30% of the genome of a hermit crab. J Biol Chem 260:1296–1303
Juan C, Petitpierre E (1991) Chromosome number and sex determining system in Tenebrionidae (Coleoptera). In: Zunino M, Belles X, Blas M (eds) Advances in coleopteorology. AEC, Barcelona, pp 167–176
Juan C, Gosalvez J, Mezzanotte R, Petitipierre E (1991) Cytological and biochemical characterization of the in situ endonuclease digestion of fixedTenebrio molitor chromosomes. Chromosoma 100: 432–438
Juan C, Vazquez P, Rubio JM, Petitpierre E, Hewitt GM (1993) Presence of highly repetitive sequences inTribolium flour-beetles. Heredity 70:1–8
Lobe AR, Roberts PA (1988) Evolution of satellite DNA sequences inDrosophila. In: Verma RS (ed) Heterochromatin. Cambridge University Press, Cambridge, MA pp 148–186
Lobe AR, Hilliker AJ, Roberts PA (1993) Mapping simple repeated DNA sequences in heterochromatin ofDrosophila melanogaster. Genetics 134:1149–1174
Martinez-Balbas A, Rodrigeuz-Campos A, Garcia-Ramirez M, Sainz J, Carrera P, Aymami J, Azorin F (1990) Satellite DNAs contain sequences that induce curvature. Biochemistry 29:2342–2348
Petitpierre E, Juan C, Pons J, Plohl M, Ugarković D (1995) Satellite DNA and constitutive heterochromatin in tenebrionid beetles. In: Kew Chromosome Conference IV. (In press)
Pinkel D, Gray J, Trask B, van den Engh G, Fuscoe J, van Dekken H (1986) Cytogenetic analysis byin situ hybridization with fluorescently labeled nucleic acid probes. Cold Spring Harb Symp Quant Biol 51:151–157
Plohl M, Borštnik B, Ugarković D, Gamulin V (1990) Sequence-induced curvature ofTenebrio mohtor satellite DNA. Biochimie 72:665–670
Plohl M, Borštnik B, Lucijanić-Justić V, Ugarković Đ (1992) Evidence for random distribution of sequence variants inTenebrio molitor satellite DNA. Genet Res 60:7–13
Plohl M, Lucijanić-Justić V, Ugarković Đ, Petitpierre E, Juan C (1993) Satellite DNA and heterochromatin of the flour beetleTribolium confusum. Genome 36:467–475
Plohl M, Ugarković D (1994a) Analysis of divergence ofAlphitobius diaperinus satellite DNA—roles of recombination, replication slip-page and gene conversion. Mol Gen Genet 242:297–304
Plohl M, Ugarković D (1994b) Characterization of two abundant satellite DNAs from the mealwormTenebrio obscurus. J Mol Evol 39:489–495
Pons J, Petitpierre E, Juan C (1993) Characterization of the heterochromatin of the darkling beetleMysolampus goudoti: cloning of two satellite DNA families and digestion of chromosomes with restriction enzymes. Hereditas 119:179–185
Radić MZ, Lundgren K, Hamkalo BA (1987) Curvature of mouse satellite DNA and condensation of heterochromatin. Cell 50:1101–1108
Rojas-Rousse D, Bigot Y, Periquet G (1993) DNA insertions as a component of the evolution of unique satellite DNA families in two genera of parasitoid wasps:Diadromus andEupelmus (Hymenoptera). Mol Biol Evol 10:383–396
Sakano H, Huppi K, Heinrich G, Tonegawa (1979) Sequences at the somatic recombination sites of immunoglobulin light-chain genes. Nature 280:288–293
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, pp. 9.16–9.19
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5476
Schmidt ER (1984) Clustered and interspersed repetitive DNA sequence family ofChironomus. The nucleotide sequence of the Claelements and of various flanking sequences. J Mol Biol 178:1–15
Simon M, Zeig J, Silverman M, Mandel G, Doolittle R (1980) Phase variation: evolution of controlling element. Science 209:1370–1374
Smith SG (1956) The status of supernumerary chromosomes in diabrotica. J Heredity 47:157–164
Sumner AT (1990) Banding with fluorochromes other than quinacrine. In: Hyman U (ed) Chromosome banding. Unwin Hyman, London, pp 163–165
Tares S, Cornuet JM, Abad P (1993) Characterization of an unusually conserved Alul highly reiterated DNA sequence family from the honeybee,Apis mellifera. Genetics 134:1195–1204
Ugarković D, Plohl M, Gamulin V (1989) Sequence variability of satellite DNA from the mealwormTenebrio molitor. Gene 83:181–183
Ugarković D, Plohl M, Lucijanić-Justić V, Borštnik B (1992) Detection of satellite DNA inPalorus ratzeburgii: analysis of curvature profiles and comparison withTenebrio molitor satellite DNA. Biochimie 74:1075–1082
Ugarković D, Petitpierre E, Juan C, Plohl M (1995) Satellite DNAs in tenebrionid species: structure, organization and evolution. Croat Chem Acta 68:627–638
Vogt P (1992) Code domains in tandem repetitive DNA sequence structures. Chromosoma 101:585–589
Author information
Authors and Affiliations
Additional information
Correspondence to: D. Ugarković
Rights and permissions
About this article
Cite this article
Ugarković, D., Durajlija, S. & Plohl, M. Evolution ofTribolium madens (Insecta, Coleoptera) satellite DNA through DNA inversion and insertion. J Mol Evol 42, 350–358 (1996). https://doi.org/10.1007/BF02337545
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02337545