Abstract
We have analyzed thenad3-rps12 locus for eight angiosperms in order to compare the utility of mitochondrial DNA and edited mRNA sequences in phylogenetic reconstruction. The two coding regions, containing from 25 to 35 editing sites in the various plants, have been concatenated in order to increase the significance of the analysis. Differing from the corresponding chloroplast sequences, unedited mitochondrial DNA sequences seem to evolve under a quasi-neutral substitution process which undifferentiates the nucleotide substitution rates for the three codon positions. By using complete gene sequences (all codon positions) we found that genomic sequences provide a classical angiosperm phylogenetic tree with a clear-cut grouping of monocotyledons and dicotyledons with Magnoliidae at the basal branch of the tree. Conversely, owing to their low nucleotide substitution rates, edited mRNA sequences were found not to be suitable for studying phylogenetic relationships among angiosperms.
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References
Bonnard G, Gualberto JM, Lamattina L, Grienenberger JM (1992) RNA editing in plant mitochondria. Crit Rev Plant Sci 10:503–524
Crane PR, Friis EM, Pedersen KR (1995) The origin and early diversification of angiosperms. Nature 374:27–33
Cronquist A (1988) The evolution and classification of flowering plants. New York Botanical Garden, New York
Felsenstein J (1993) PHYLIP (Phylogeny Inference Package). Distributed by the author, Department of Genetics, University of Washington, Seattle
GCG (1994) Program manual for the GCG package, 575 Science Drive, Madison, Wisconsin, USA 53711
Gualberto JM, Wintz H, Weil JH, Grienenberger JM (1988) The genes coding for subunit 3 of NADH dehydrogenase and for ribosomal protein S12 are present in the wheat and maize mitochondrial genomes and are cotranscribed. Mol Gen Genet 215:118–127
Hiesel RA, Von Haeseler A, Brennicke A (1994) Plant mitochondrial nucleic acid sequences as a tool for phylogenetic analysis. Proc Natl Acad Sci USA 21:634–638
Janke A, Feldmaier-Fuchs G, Thomas W, von Haesler A, Pääbo S (1994) The marsupial mitochondrial genome and evolution of placental mammals. Genetics 137:243–256
Karpinska B, Karpinski S, Hallgren JE (1995) The genes encoding subunit 3 of NADH dehydrogenase and ribosomal protein S12 are co-transcribed and edited in Pious sylvestris (L.) mitochondrial. Curr Genet 28:423–428
Kim KS, Schuster W, Brennicke A, Choi K-T (1991) Korean ginseng mitochondrial DNA encodes an intact rps 12 gene downstream of the nad3 gene. Plant Physiol 97:1602–1603
Malek O, Lattig K, Hiesel R, Brennicke A, Knoop V (1996) RNA editing in bryophytes and a molecular phylogeny of land plants. EMBO J 15:1403–1411
Martin W, Lydiate D, Brinkmann H, Forkmann G, Saedler H, Cerff R (1993) Molecular phylogenies in Angiosperm evolution. Mol Biol Evol 10:140–162
Perrotta G, Regina TMR, Ceci LR, Quagliariello C (1996) Conserved organization of the mitochondrialnad3 andrps12 genes over evolutionary distant angiosperms. Mol Gen Genet 251:326–337
Pring D, Brennicke A, Schuster W (1993) RNA editing gives a new meaning to the genetic information in mitochondria and chloroplast. Plant Mol Biol 21:1163–1170
Rasmussen J, Hanson MR (1989) A NADH dehydrogenase subunit gene is cotranscribed with the abnormalPetunia mitochondrial gene associated with cytoplasmic male sterility. Mol Gen Genet 215:332–336
Saccone C, Lanave C, Pesole G, Preparata G (1990) Influence of base composition on quantitative estimates of gene evolution. Methods Enzymol 183:570–583
Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
Schuster W, Unseld M, Wissinger B, Brennicke A (1990) Transcripts of the NADH-dehydrogenase subunit 3 gene are differentially edited inOenothera mitochondrial. EMBO 19:263–269
Suzuki T, Kazama S, Hirai A, Akihama T, Kadowaki K (1991) The rice mitochondrialnad3 gene has an extended reading frame at its 5′ end: nucleotide sequence analysis of ricetrnS, nad3 andrps12 genes. Curr Genet 20:331–337
Wolfe KH, Li W-H, Sharp PM (1987) Rates of nucleotide substitutions vary greatly among plant mitochondrial, chloroplast, and nuclear DNAs. Proc Natl Acad Sci USA 84:9054–9058
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Correspondence to: G. Pesole
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Pesole, G., Ceci, L.R., Gissi, C. et al. Evolution of thenad3-rps12 gene cluster in angiosperm mitochondria: Comparison of edited and unedited sequences. J Mol Evol 43, 447–452 (1996). https://doi.org/10.1007/BF02337516
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DOI: https://doi.org/10.1007/BF02337516