Abstract
In order to be considered usable as synthetic seeds, encapsulated explants sown underin vitro orex vitro conditions must be able to produce whole plantlets. Ninety percent of non-encapsulated M.26 apple rootstock single nodes produced a plantlet (i.e., a well-formed shoot with a root system) after 30 days of culturein vitro if the explants were previously given a 24-hour treatment with 24.6 µM IBA and 15 g 1−1 sucrose in darkness. In contrast, when the single nodes were encapsulated in a calcium-sodium alginate bead immediately after the same treatment only 10% of the encapsulated explants formed a plantlet. Addition of growth regulators to the artificial endosperm and culture of the single nodes for root primordia initiation for 3, 6 or 9 days in darkness before encapsulation allowed production of 58%, 60% and 66% of plantlets, respectively.
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Abbreviations
- AdSO4 :
-
adenine hemisulfate
- BA:
-
benzyladenine
- GA3 :
-
gibberellic acid
- IBA:
-
indolebutyric acid
- PVP:
-
polyvinyl pyrrolidone
- Synseed:
-
synthetic seed
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Piccioni, E. Plantlets from encapsulated micropropagated buds of M.26 apple rootstock. Plant Cell Tiss Organ Cult 47, 255–260 (1997). https://doi.org/10.1007/BF02318980
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DOI: https://doi.org/10.1007/BF02318980