Abstract
The endogenous levels of isopentenyladenine, isopentenyladenosine, zeatin and zeatin riboside and the ability forin vitro axillary shoot organogenesis and rhizogenesis were compared between mature and rejuvenated clones ofHevea brasiliensis (Müll. Arg.). Enhancement of thein vitro organogenesis ability of rubber-tree clones following somatic embryogenesis or repeated grafting onto juvenile rootstocks was accompanied by an increase of zeatin riboside levels in shoots used as starting material forin vitro micropropagation. Furthermore, the zeatin level, inin vitro shoots of clones treated byin vitro micrografting, and consequently capable of axillary shoot and root organogenesis, was higher than inin vitro shoots of non treated mature material incapable of in vitro organogenesis. We conclude that the endogenous zeatin-like cytokinin level (free and ribosylated forms) can be considered as a reliable marker for the recovery ofin vitro shoot and root organogenesis after rejuvenating treatments in rubber-tree clones.
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Abbreviations
- ZR:
-
zeatin riboside
- 2iP:
-
isopentenyladenine
- iPA:
-
isopentenyladenosine
- NAA:
-
α-naphthaleneacetic acid
- BA:
-
6-benzyladenine
- IBA:
-
indole-3-butyric acid
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Perrin, Y., Doumas, P., Lardets, L. et al. Endogenous cytokinins as biochemical markers of rubber-tree (Hevea brasiliensis) clone rejuvenation. Plant Cell Tiss Organ Cult 47, 239–245 (1997). https://doi.org/10.1007/BF02318978
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DOI: https://doi.org/10.1007/BF02318978