Summary
The amino acid sequences of the pancreatic ribonuclease from capybara (Hydrochoerus hydrochaeris) and cuis (Galea musteloides) were determined. Both species belong to the same superfamily of the hystricomorph rodents as the guinea-pig. In guineapig pancreas two ribonucleases are present as a result of a rencent gene duplication, but in capybara and cuis pancreas only one single ribonuclease has been found. A most parsimonious tree of ribonucleases indicates that the gene duplication leading to both guinea-pig ribonucleases occurred before the divergence of guineapig from capybara and cuis. This would mean that changes in expression of the ribonuclease genes have occurred in these taxa.
Cuis and capybara ribonuclease have no Asn-X-Ser/Thr sequences and are carbohydrate-free proteins. Capybara ribonculease has leucine at position 114, a position occupied by proline in thecis-configuration in bovine pancreatic ribonuclease.
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Data supplementary to this article are deposited with, and can be obtained from Elsevier Biomedical Press B.V., BBA Data Deposition, P.O. Box 1345, 1000 BH Amsterdam, The Netherlands. Reference should be made to BBA/DD/246. The supplementary information included Tables 3 and 4 (procedures used for purification of peptides from capybara and cuis ribonuclease and charge determinations of peptides at pH 6.5), Tables 5 and 6 (amino acid compositions of peptides) and Figs. 5–9 (gel filtration patterns of CNBr and enzymic digests).
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Beintema, J.J., Neuteboom, B. Origin of the duplicated ribonuclease gene in guinea-pig: Comparison of the amino acid sequences with those of two close relatives: Capybara and cuis ribonuclease. J Mol Evol 19, 145–152 (1983). https://doi.org/10.1007/BF02300752
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DOI: https://doi.org/10.1007/BF02300752