Abstract
Well-defined G-bands were obtained on metaphase chromosomes fromTapinoma nigerrimum using trypsin and warm 2×SSC in sequence. The G-banded pattern allowed the identification of all chromosomes. Evidence for asynchronous condensation of the chromosomes of this species is provided. Different banding patterns were obtained when metaphase chromosomes were stained with DA/DAPI alone and with DA/DAPI after a standard G-banding procedure. The G-banding phenomenon is discussed using the result obtained.
Article PDF
Avoid common mistakes on your manuscript.
References
Babu A, Verma RS (1987) Chromosome structure: euchromatin and heterochromatin.Int Rev Cytol 108: 1–60.
Bella JL, Gosálvez J (1991) C-banding with specific fluorescent DNA-ligands: a new approach to constitutive heterochromatin heterogeneity.Biotech Histochem 1: 44–52.
Bella JL, Gosálvez J (1994) Banding human chromosomes using a combined C-banding-fluorochrome staining technique.Biotech Histochem 69: 243–248.
Burgos M, Jiménez R, Díaz de la Guardia R (1986) A rapid, simple and reliable combined method for G-banding mammalian and human chromosomes.Stain Technol 61: 257–260.
Crozier RH (1975) Hymenoptera. In: John B, ed.Animal Cytogenetics, Vol. 3Insecta 7. Berlin: Gebrüger Borntraeger.
Hirai H, Yamamoto MT, Ogura K et al. (1994) Multiplication of 28S rDNA and NOR activity in chromosome evolution among ants of theMyrmecia pilosula species complex.Chromosoma 103: 171–178.
Holmquist, GP (1989) Evolution of chromosome bands: molecular ecology of non-coding DNA.J Mol Evol 28: 469–486.
Imai HT, Taylor RW, Crozier RH (1994) Experimental bases for the minimum interaction theory. I. Chromosome evolution in ants of theMyrmecia pilosula species complex (Hymenoptera: Formicidae: Myrmeciinae).Jpn J Genet 69: 137–182.
Isacchi A, Bernardi G, Bernardi G (1993) Compositional compartmentalization of the nuclear genomes ofTrypanosoma brucei andTrypanosoma equiperdum.FEBS Lett 335: 181–183.
Kakeda K, Fukui K (1994) Dynamic changes in the morphology of barley chromosomes during the mitotic metaphase stage.Jpn J Genet 69: 545–554.
Meredith R (1969) A simple method for preparing meiotic chromosomes from mammalian testis.Chromosoma 26: 254–258.
Odierna G, Baldanza F, Aprea G, Olmo E (1993) Occurrence of G-banding in metaphase chromosomes ofEncarsia berlesei (Hymenoptera: Aphelinidae).Genome 36: 662–667.
Palomeque T, Chica E, Cano MA, Díaz de la Guardia R (1988) Karyotypes, C-banding, and chromosomal location of active nucleolar organizing regions inTapinoma (Hymenoptera, Formicidae).Genome 30: 277–280.
Palomeque T, Chica E, Cano MA, Díaz de la Guardia R (1990) Development of silver stained structures during spermatogenesis in different genera of Formicidae.Genetica 81: 51–58.
Palomeque T, Chica E, Díaz de la Guardia R (1993) Supernumerary chromosome segments in different genera of Formicidae.Genetica 90: 17–29.
Ponce de Leon FA, Li Y, Weng Z (1992). Early and late replicative chromosomal banding patterns ofGallus domesticus.J Hered 83: 36–42.
Prosperi E, Giangarè MC, Bottiroli G (1994) DNA stainability with base-specific fluorochromes: dependence on the DNA topologyin situ.Histochemistry 102: 123–128.
Schweizer D (1980) Simultaneous fluorescent staining of R bands and specific heterochromatin regions (DA-DAPI bands) in human chromosomes.Cytogenet Cell Genet 27: 190–193.
Sumner AT (1994) Functional aspects of the longitudinal differentiation of chromosomes.Eur J Histochem 38: 91–109.
Warchalowska-Sliwa E, Maryanska-Nadachowska A, Jordan M (1978) C-and G-banding in mitotic chromosomes ofAcheta domesticus (Orthoptera).Folia Biol 26: 295–306.
Wolf KW, Blackman RL, Summer AT (1994) Scanning electron microscopy of insect chromosomes.Schistocerca gregaria (Acrididae, Orthoptera),Megaselia scalaris (Phoridae, Diptera), andMyzus persicae (Aphididae, Hemiptera).J Submicrosc Cytol Pathol 26: 79–89.
Author information
Authors and Affiliations
Additional information
accepted for publication by H. C. Macgregor
Rights and permissions
About this article
Cite this article
Lorite, P., Chica, E. & Palomeque, T. G-banding and chromosome condensation in the ant,Tapinoma nigerrimum . Chromosome Res 4, 77–79 (1996). https://doi.org/10.1007/BF02254949
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02254949