Abstract
We have investigated whole-cell Ba2+ currents through Ca2+ channels (IBa) in single myocytes freshly isolated from the aortic media of neonatal (1-day-old) and adult (12-week-old) rats. In neonatal myocytes, IBa was undetectable even in presence of the dihydropyridine (DHP) agonist Bay K 8644. Binding of [3H]Nitrendipine on crude plasma membrane preparation of media confirmed the absence of DHP-receptors. By contrast, a robust DHP-sensitive ‘L-type’ IBa was recorded in adults which was consistent with the presence of specific [3H]Nitrendipine binding sites. In conclusion, neonatal aortic myocytes do not express any Ca2+ channels. The acquisition of L-type Ca2+ channels may be related to cell differentiation and acquisition of contractility during postnatal development.
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Quignard, JF., Grazzini, E., Guillon, G. et al. Absence of calcium channels in neonatal rat aortic myocytes. Pflugers Arch. 431, 791–793 (1996). https://doi.org/10.1007/BF02253845
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DOI: https://doi.org/10.1007/BF02253845