Abstract
A single prior undrugged exposure to the elevated plus-maze has been reported to reduce open arm activity on retest and to attenuate/abolish the anxiolytic response to benzodiazepines at retest intervals ranging from 48 h to 14 days. The present study was designed to examine the generality of these findings by comparing the effects of prior maze experience on baseline behaviour and response to diazepam in two murine models of anxiety. Parallel experiments were conducted in which DBA/2 mice were exposed/not exposed to the plus-maze, treated daily with saline or diazepam (2–4 mg/kg daily for 8 days) and then tested on either the elevated plus-maze or in the light/dark test of exploration. Results show that, in both tests, diazepam reduced behavioural indices of anxiety in maze-naive mice only. However, interpretation of this apparent loss of diazepam efficacy is at least partially confounded by the observation that maze experience per se altered baseline behaviour in both procedures,reducing open arm activity in the plus-maze andincreasing light compartment activity in the light/dark test. The apparent elimination of an anxiolytic response to diazepam in two animal models of anxiety by prior plus-maze experience is discussed in relation to experience-related baseline shifts in behaviour.
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References
Almeida SS, Garcia RA, DeOliveira LM (1993) Effects of early protein malnutrition and repeated testing upon locomotor and exploratory behaviors in the elevated plus-maze. Physiol Behav (in press)
Barry JM, Costall B, Kelly ME, Naylor RJ, Onaivi ES (1987) A simple habituation test in the mouse. Br J Pharmacol 92:651P
Benjamin D, Lal H, Meyerson LR (1990) The effects of 5-HT1B characterizing agents in the mouse elevated plus-maze. Life Sci 47:195–203
Costall B, Jones BJ, Kelly ME, Naylor RJ, Tomkins DM (1989) Exploration of mice in a black and white test box: validation as a model of anxiety. Pharmacol Biochem Behav 32:777–785
Crawley JN, Goodwin FK (1981) Neuropharmacologic specificity of a simple animal model for the behavioural actions of benzodiazepines. Pharmacol Biochem Behav 15:695–699
Critchley MAE, Njung'e K, Handley SL (1992) Actions and some interactions of 5-HT1A ligands in the elevated X-maze and effects of dorsal raphe lesions. Psychopharmacology 106:484–490
DaCunha C, DeStein ML, Wolfman C, Koya R, Izquierdo I, Medina JH (1992) Effect of various training procedures on performance in an elevated plus-maze: possible relation with brain regional levels of benzodiazepine-like molecules. Pharmacol Biochem Behav 43:677–681
File SE (1990) One-trial tolerance to the anxiolytic effects of chlordiazepoxide in the plus-maze. Physchopharmacology 100:281–282
File SE, Hitchcott PK (1990) A theory of benzodiazepine dependence that can explain whether flumazenil will enhance or reverse the phenomena. Psychopharmacology 101:525–532
File SE, Zangrossi H (1993) “One trial tolerance” to the anxiolytic actions of benzodiazepines in the elevated plus-maze, or the development of a phobic state? Physchopharmacology 110:240–244
File SE, Mabbutt PS, Hitchcott PK (1990) Characterisation of the phenomenon of “one-trial tolerance” to the anxiolytic effect of chlordiazepoxide in the elevated plus-maze. Psychopharmacology 102:98–101
File SE, Andrews N, Wu PY, Zharkovsky A, Zangrossi H (1992) Modification of chlordiazepoxide's behavioural and neurochemical effects by handling and plus-maze experience. Eur J Pharmacol 218:9–14
Itoh J, Nabeshima T, Kameyama T (1990) Utility of an elevated plus-maze for the evaluation of memory in mice: effects of nootropics, scopolamine and electroconvulsive shock. Psychopharmacology 101:525–532
Lee C, Rodgers RJ (1990) Antinociceptive effects of the elevated plus-maze: influence of opiate receptor manipulations. Psychopharmacology 102:507–513
Lister RG (1987) The use of a plus-maze to measure anxiety in the mouse. Psychopharmacology 92:180–185
Lister RG (1990) Ethologically-based animal models of anxiety disorders. Pharmacol Ther 46:321–340
Montgomery KC (1955) The relation between fear induced by novelty stimulation and exploratory behaviour. J Comp Physiol Psychol 48:254–260
Moser PC, Tricklebank MD, Middlemiss DN, Mir AK, Hibert MF, Fozard JR (1990) Characterization of MDL 73005EF as a 5-HT1A selective ligand and its effects in animal models of anxiety: comparison with buspirone, 8-OH-DPAT and diazepam. Br J Pharmacol 99:343–349
Nutt DJ (1990) The pharmacology of human anxiety. Pharmacol Ther 47:233–236
Onaivi ES, Martin BR (1989) Neuropharmacological and physiological validation of a computer-controlled two-compartment black and white box for the assessment of anxiety. Prog Neuropsychopharmacol Biol Psychiatry 13:963–976
Pellow S, File SE (1986) Anxiolytic and anxiogenic drug effects on exploratory activity in an elevated plus-maze: a novel test of anxiety in the rat. Pharmacol Biochem Behav 24:525–529
Pellow S, Chopin P, File SE, Briley M (1985) Validation of open: closed arm entries in an elevated plus-maze as a measure of anxiety in the rat. J Neurosci Methods 14:149–167
Rodgers RJ, Cole JC, (1993) Influence of social isolation, gender, strain and prior novelty on plus-maze behaviour in mice. Physiol Behav (in press)
Rodgers RJ, Cole JC Cobain M, Daly P, Doran PJ, Eells JR, Wallis P (1992a) Anxiogenic-like effects of fluprazine and eltoprazine in the mouse elevated plus-maze: profile comparisons with 8-OH-DPAT, CGS 12066B, TFMPP and mCPP. Behav Pharmacol 3:621–634
Rodgers RJ, Lee C, Shepherd JK (1992b) Effects of diazepam on behavioural and antinociceptive responses to the elevated plusmaze in male mice depend upon treatment regimen and prior maze experience. Psychopharmacology 106:102–110
Shepherd JK (1992) Preliminary evaluation of an elevated “zeromaze” as an animal model of anxiety in laboratory rats. J Psychopharmacol Abstracts, A56
Treit D, Menard J, Royan C (1993) Anxiogenic stimuli in the elevated plus-maze. Pharmacol Biochem Behav 44:463–469
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Rodgers, R.J., Shepherd, J.K. Influence of prior maze experience on behaviour and response to diazepam in the elevated plus-maze and light/dark tests of anxiety in mice. Psychopharmacology 113, 237–242 (1993). https://doi.org/10.1007/BF02245704
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DOI: https://doi.org/10.1007/BF02245704