Abstract
Extrathymic pathways for T cell differentiation were recently demonstrated in the liver, intestine and omentum. In this study, glycyrrhizin (GL), a plant extract was investigated as to its effect on extrathymic T cells in the liver of mice. A new method using anti-LFA-1 mAb in conjunction with anti-TCR or -CD3 mAbs to sensitively identify such extrathymic T cells is included. Single injection and repeated injections of GL increased not only the number of total hepatic MNC but also the proportion of intermediate TCR cells, which are extrathymic T cells uniquely seen in the liver. In contrast to other tested reagents (e.g., lymphotoxin and estrogen) that activated the extrathymic T cells and simultaneously induced profound thymic atrophy, GL did not affect regular T cells in the thymus. The present results suggest that the selective activation of extrathymic T cells in the liver might be intimately related to the clinical effects of GL.
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Abbreviations
- GL:
-
glycyrrhizin
- TCR:
-
T cell receptor
- mAb:
-
monoclonal antibody
- DP:
-
double-positive
- DN:
-
double-negative
- MHC:
-
major histocompatibility complex
- MNC:
-
mononuclear cells
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Kimura, M., Watanabe, H. & Abo, T. Selective activation of extrathymic T cells in the liver by glycyrrhizin. Biotherapy 5, 167–176 (1992). https://doi.org/10.1007/BF02171049
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DOI: https://doi.org/10.1007/BF02171049