Summary
The tetranucleoside triphosphoramidates CNHpGNHpCNHpGN 3 and GNHpCNHpGNHpCN 3 do not affect the rate of condensation between dinucleoside phosphoramidates pCNHpGN 3 and CNHpGNH 2 in the presence of a water-soluble carbodiimide. This result contrasts with the previously reported efficient template-directed oligomerization of the corresponding GC dimers on the phosphoramidate-linked tetramer of sequence GCGC. These findings are discussed in terms of the relative stability of helices formed by the template itself and by the template with complementary dimers.
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Abbreviations
- Hepes:
-
4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid
- EDAC:
-
1-ethyl-3-(dimethylaminopropyl)-carbodiimide hydrochloride
- CNHpGNH 2 :
-
dinucleoside (3′→5′) phosphoramidate having an amino group at the 3′-end
- pCNHpGN 3′ :
-
5′-phosphorylated dinucleoside (3′–5′) phosphoramidate having an azido group at the 3′-end
- HPLC:
-
high performance liquid chromatography
References
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Zielinski, W.S., Orgel, L.E. The template properties of tetranucleoside triphosphoramidates having cytosine-guanosine residues. J Mol Evol 29, 281–283 (1989). https://doi.org/10.1007/BF02103614
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DOI: https://doi.org/10.1007/BF02103614