Summary
We have analyzed ∼18 kb of DNA in and upstream of thedefective chorion-1 (dec-1) locus of the eight known species of themelanogaster species subgroup ofDrosophila. The restriction maps ofD. simulans, D. mauritiana, D. sechellia, D. erecta, andD. orena are shown to have basically the restriction map ofD. melanogaster, whereas the maps ofD. teissieri andD. yakuba were more difficult to align. However, the basic amount of DNA and sequence arrangement appear to have been conserved in these species. A small deletion of varying length (65–200 bp) is found in a repeated sequence of the central transcribed region ofD. melanogaster, D. simulans, andD. erecta. Restriction site mapping indicated that thedec-1 gene is highly conserved in themelanogaster species subgroup. However, sequence comparison revealed that the amount of nucleotide and amino acid substitution in the repeated region is much larger than in the 5′ translated region. The 5′ flanking region showed noticeable restriction site polymorphisms between species. Based on calculations from the restriction maps a dendrogram was derived that supports earlier published phylogenetic relationships within themelanogaster species subgroup except that theerecta-orena pair is placed closer to themelanogaster complex than toD. teissieri andD. yakuba.
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References
Ashburner M, Bodmer M, Lemeunier F (1984) On the evolutionary relationships ofDrosophila melanogaster. Dev Genet 4:295–312
Barrie PA, Jeffreys AJ, Scott AF (1981) Evolution of the β-globin gene cluster in man and primates. J Mol Biol 149:319–336
Bauer BJ, Waring GL (1987) 7C female sterile mutants fail to accumulate early eggshell proteins necessary for later chorion morphogenesis inDrosophila. Dev Biol 121:349–358
Bishop JG, Hunt JA (1988) DNA divergence in and around the alcohol dehydrogenase locus in five closely related species of HawaiianDrosophila. Mol Biol Evol 5:415–431
Bodmer M, Ashburner M (1984) Conservation and change in the sequences coding for alcohol dehydrogenase in sibling species ofDrosophila. Nature 309:425–430
Brennan MD, Weiner AJ, Goralski TJ, Mahowald AP (1982) The follicle cells are a major site of vitellogenin synthesis inDrosophila melanogaster. Dev Biol 89:225–236
Caccone A, Amato D, Powell JR (1988) Rates and patterns of scnDNA and mtDNA divergence within theDrosophila melanogaster subgroup. Genetics 118:617–683
Daïnou O, Cariou ML, David JR, Hickey D (1987) Amylase gene duplication: an ancestral trait in theDrosophila melanogaster species subgroup. Heredity 59:245–251
Fenerjian MG, Martínez-Cruzado JC, Swimmer C, King D, Kafatos FC (1989) Evolution of the autosomal chorion cluster inDrosophila. II. Chorion gene expression and sequence comparisons of thes16 ands19 genes in evolutionarily distant species. J Mol Evol 29:108–125
Griffin-Shea R, Thireos G, Kafatos FC (1982) Organization of a cluster of four chorion genes inDrosophila and its relationship to developmental expression and amplification. Dev Biol 91:325–336
Hawley RJ, Waring GL (1988) Cloning and analysis of thedec-1 female-sterile locus, a gene required for proper assembly of theDrosophila eggshell. Genes & Dev 2:341–349
Jowett T (1986) Preparation of nucleic acids. In: Roberts DB (ed)Drosophila, a practical approach. IRL Press Oxford, Washington DC, pp 276–285
Kassis JA, Desplan C, Wright DK, O'Farrel PH (1989) Evolutionary conservation of homeodomain-binding sites and other sequences upstream and within the major transcription unit of theDrosophila segmentation gene engrailed. Mol Cell Biol 9:4304–4311
Keller BK, Noon WA (1985) Intron splicing: a conserved internal signal in introns ofDrosophila pre-mRNAs. Nucleic Acids Res 13:4971–4981
King RC, Koch EA (1963) Studies on the ovarian follicle cells ofDrosophila. Q J Microsc Sci 104:297–320
Komitopoulou K, Margaritis LH, Kafatos FC (1988) Structural and biochemical studies on four sex-linked chorion mutants ofDrosophila melanogaster. Dev Genet 9:37–48
Lachaise D, Cariou ML, David JR, Lemeunier F, Tsacas L, Ashburner M (1988) Historical biogeography of theDrosophila melanogaster species subgroup. Evol Biol 22:159–225
Langley CH, Montgomery E, Quattlebaum WF (1982) Restriction map variation in theAdh region ofDrosophila. Proc Natl Acad Sci USA 79:5631–5635
Leigh-Brown AJ, Ish-Horowitz D (1981) Evolution of the 87A and 87C heat-shock loci inDrosophila. Nature 290:677–682
Lineruth K (1987) An ovarian protein locus and its developmentally regulated expression inDrosophila Doctoral thesis, University of Umeå, Sweden
Lineruth K (1989) Synthesis patterns of a set of follicle cell proteins inDrosophila melanogaster sibling species. Genetica 78:33–37
Lineruth K, Lambertsson A (1985) Stage specific synthesis of some follicle cell proteins inDrosophila melanogaster. Wilhelm Roux's Arch Dev Biol 194:436–439
Lineruth K, Lambertsson A (1986) Correlation between a female sterile mutation and a set of follicle cell proteins inDrosophila melanogaster. Mol Gen Genet 205:213–216
Lineruth K, Lindberg M (1988) Electrophoretic variant forms of a set of follicle cell proteins inDrosophila melanogaster: frequencies in wild-type strains and response in a selection experiment. Hereditas 108:59–64
Lineruth K, Lambertsson A, Lindberg M (1985) Genetic localization of a follicle cell protein locus inDrosophila melanogaster. Mol Gen Genet 201:375–378
Margaritis LH (1985) Structure and physiology of the eggshell. In: Kerkut GA, Gilbert LI (eds) Comprehensive insect physiology, biochemistry, and pharmacology, vol 1. Pergamon Press, Elmsford NY, pp 153–230
Margaritis LH, Kafatos FC, Petri WH (1980) The eggshell ofDrosophila melanogaster. I. Fine structure of the layers and regions of the wild-type eggshell. J Cell Sci 43:1–35
Martin CH, Meyerowitz EM (1986) Characterization of the boundaries between adjacent rapidly and slowly evolving genomic regions inDrosophila. Proc Natl Acad Sci USA 83:8654–8658
Martin CH, Mayeda CA, Meyerowitz EM (1988) Evolution and expression of theSgs-3 glue gene ofDrosophila. J Mol Biol 201:273–287
Martínez-Cruzado JC, Swimmer C, Fenerjian MG, Kafatos FC (1988) Evolution of the autosomal chorion locus inDrosophila. I. General organization of the locus and sequence comparisons of genes s15 and s19 in evolutionarily distant species. Genetics 119:663–677
Meyerowitz EM, Martin CH (1984) Adjacent chromosomal regions can evolve at very different rates: evolution of theDrosophila 68C glue gene cluster. J Mol Evol 20:251–264
Mount SM (1982) A catalogue of splice junction sequences. Nucleic Acids Res 10:459–472
Nei M, Li W-H (1979) Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA 76:5269–5273
Parks S, Wakimoto B, Spradling AC (1986) Replication and expression of an X-linked cluster ofDrosophila chorion genes. Dev Biol 117:294–305
Petri WH, Wyman AR, Kafatos FC (1976) Specific protein synthesis in cellular differentiation. III. The eggshell proteins ofDrosophila melanogaster and their program of synthesis. Z Zellforsch 95:495–510
Saitou N, Nei M (1987) The neighbor-joining method: a new method for constructing phylogenetic trees. Mol Biol Evol 4:406–425
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467
Spradling AC (1981) The organization and amplification of two clusters ofDrosophila chorion genes. Cell 27:193–202
Spradling AC, Mahowald AP (1980) Amplification of genes for chorion proteins during oogenesis inDrosophila melanogaster. Proc Natl Acad Sci USA 77:1096–1100
Spradling AC, Digan ME, Mahowald AP, Scott M, Craig EA (1980) Two clusters of genes for major chorion proteins ofDrosophila melanogaster. Cell 19:905–914
Waring GL, Mahowald AP (1979) Identification and time of synthesis of chorion proteins inDrosophila melanogaster. Cell 16:599–607
Waring GL, Hawley RJ, Schoenfeld T (1990) Multiple proteins are produced from thedec-1 eggshell gene inDrosophila by alternative RNA splicing and proteolytic cleavage events. Dev Biol 142:1–12
Zwiebel LJ, Cohn VH, Wright DR, Moore GP (1982) Evolution of single-copy DNA and the ADH gene in seven drosophilids. J Mol Evol 19:62–71
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Andersson, S., Lambertsson, A. Evolution of thedec-1 eggshell locus inDrosophila. I. Restriction site mapping and limited sequence comparison in themelanogaster species subgroup. J Mol Evol 33, 321–331 (1991). https://doi.org/10.1007/BF02102863
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DOI: https://doi.org/10.1007/BF02102863