Summary
Interaction based on possible chemical affinity of an amino acid for tRNA was examined as a model for the aminoacylation of primitive tRNA without aid of an enzyme system. Two types of reaction were carried out and compared. One was the acyl linkage of amino acid to the 5′-terminal phosphate of a tRNA activated as an imidazolide. The other was the incorporation of an amino acid activated as an imidazolide into 2′(3′)-hydroxyl groups of intact tRNA. Both types of reaction indicated that none of the amino acids tested had any selectivity for the tRNAs examined. However, the rates of reaction with a given tRNA were different among amino acids. In the second type of reaction, amino acids were found mainly at loop-out regions of tRNA, but not at either its 5′- or 3′-terminal sites
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Abbreviations
- AA-Im:
-
aminoacyl imidazolide
- 2′(3′)-O-AA-tRNA:
-
2′(3′)-O-aminoacyl-tRNA
- 5′-O-AA-tRNA:
-
phosphate ester of amino acid at the 5′-terminal phosphate of tRNA
- CDI:
-
N,N′-carbonyldiimidazole
- DMF:
-
dimethylformamide
- DMSO:
-
dimethylsulfoxide
- EDTA:
-
ethylenediaminetetraacetic acid
- TFA:
-
trifluoroacetic acid
- Im-tRNA:
-
5′-imidazolylphosphonate of tRNA
References
Crothers DM, Seno T, Soll DG (1972) Is there a discriminator site in transfer RNA? Proc Natl Acad Sci USA 69:3063–3067
Ehler KW, Orgel LE (1976) N,N′-carbonyldiimidazole-induced peptide formation in aqueous solution. Biochim Biophys Acta 434:233–243
Ehler KW, Girard E, Orgel LE (1977) Reaction of polyfunctional amino acid with N,N′-carbonyldiimidazole in aqueous solution—oligopeptide formation. Biochim Biophys Acta 491: 253–264
Gillam I, Milward S, Blew D, von Tigerstorm M, Wimmer E, Tenner GM (1967) The separation of soluble ribonucleic acid on benzoylated diethylaminoethylcellulose. Biochemistry 6:3043–3056
Gottikh BP, Krayevsky AA, Tarussova NB, Purygin PP, Tsilevich TL (1970) The general synthetic route to amino acid esters of nucleotide and nucleotide 5′-triphosphates and some properties of these compounds. Tetrahedron 26:4419–4433
Hasegawa T, Ishikura H (1978) Nucleotide sequence of threonine tRNA fromBacillus subtilis. Nucleic Acids Res 5:537–548
Holmes WM, Hurd RE, Reid BR, Rimerman RA, Hatfield GW (1975) Separation of transfer ribonucleic acid by Sepharose chromatography using reverse salt gradients. Proc Natl Acad Sci USA 72:1068–1071
Ishigami M, Nagano K, Tonotsuka N (1977) The origin of the protein synthesis mechanism. Biosystems 9:229–243
Jencks WP, Carriuolo J (1959) Imidazole catalysis. J Biol Chem 243:1272–1279
Johes AS (1953) The isolation of bacterial nucleic acids using cetyltrimethylammonium bromide (CETAVLON). Biochim Biophys Acta 10:607–612
Lohrman R, Ranganathan R, Sawai H, Orgel LE (1975) Prebiotic peptide-formation in the solid state. I. Reactions of benzoate ion and glycine with adenosine 5′-phosphorimidazolide. J Mol Evol 5:57–73
Mukaiyama T, Hashimoto M (1971) Phosphorylation by oxidation-reduction condensation. Preparation of active phosphorylating reagents. Bull Chem Soc Japan 44:2284
Sanger F, Brownlee GG, Barrell BG (1965) A two dimensional fractionation procedure for radioactive nucleotide. J Mol Biol 13:373–398
Vold BS (1973) Analysis of isoaccepting transfer ribonucleic acid species ofBacillus subtilus: chromatographic differences between transfer ribonucleic acid from spores and cell in exponential growth. J Bacteriol 113:825–833
Weber AL, Fox SW (1973) Aminoacylation and acetylaminoacylation of homopolyribonucleotides. Biochim Biophys Acta 319:174–187
Weber AL, Lacey JC Jr (1975) Aminoacyl transfer from an adenylate anhydride to polynucleotides. J Mol Evol 6:309–320
Weber AL, Lacey JC Jr (1978) Genetic code correlations: amino acids and their anticodon nucleotides. J Mol Evol 11:199–210
White WE Jr, Laccy JC Jr, Weber AL (1973) Aminoacyl transfer from adenylate anhydride to the 2′ OH group along the backbone of polyribonucleotides. Biochem Biophys Res Commun 51:283–291
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OneA 260 unit is defined as an amount of material which gives an absorption of 1.0 at 260 nm when dissolved in 1 ml water and measured with a 1-cm light path
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Kinjo, M., Ishigami, M., Hasegawa, T. et al. Differential coupling efficiency of chemically activated amino acid to tRNA. J Mol Evol 20, 59–65 (1984). https://doi.org/10.1007/BF02101986
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DOI: https://doi.org/10.1007/BF02101986